PP2A beta protein-GST fusion

Application Note: PP2Aβ Protein is stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol. PP2Aβ Protein is suitable for use in Western Blot. Expect a band approximately ~59kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Concentration Value: 0.1 µg/µL

Western Blot Dilution: User Optimized

General Disclaimer Note: This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Physical State: Liquid (sterile filtered)

Purity and Specificity: Recombinant full-length human PP2Aβ was expressed by baculovirus in Sf9 insect cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >75% by densitometry.

Background: PP2A is one of the four major Ser/Thr phosphatases which are implicated in the negative control of cell growth and division and encodes a specific phosphotyrosyl phosphatase activator of the dimeric form of protein phosphatase 2A (1). PP2A holoenzymes are heterotrimeric proteins composed of a structural subunit A, a catalytic subunit C, and a regulatory subunit B which is encoded by a diverse set of genes. The interaction was independent of ATP or magnesium ion and the inclusion of PTPA altered the substrate specificity of PP2A, with enhanced phosphotyrosine phosphatase activity and decreased phosphoserine phosphatase activity (2). PP2Aβ Protein is ideal for investigators involved in Signaling Proteins, Cellular Proteins, Cancer, Cardiovascular Disease, Cell Cycle, ERK/MAPK Pathway, Metabolic Disorder, Phosphatases, Ser/Thr Kinases, and WNT Signaling research.

Low Endotoxin: No

• Artikelnummer: ROC009-001-T01S
• Hersteller: Rockland
• Hersteller Artikelnummer: 009-001-T01S
• Verpackungseinheit: 20 µg
• Mengeneinheit: STK
• Konjugat: Unconjugated