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Praja1, SUMO-tag Recombinant
Praja1, SUMO-tag Recombinant
Artikelnummer
BPS81055
Verpackungseinheit
25 µg
Hersteller
BPS Bioscience
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Encompassing Amino Acids: full length
Applications: Useful as an E3 ligase in ubiquitin-conjugating reactions and to analyze upregulation/downregulation of E3 enzymes.
Background: Praja1 (along with Praja2, 53% identity) is a RING-H2 domain E3 (ubiquitin protein ligase) that is involved in the conjugation of ubiquitin to target substrates along with E1 and E2 enzymes. Substrates for Praja1 include Dlxin-1 (MAGE-D1, human) which regulates Dlx5 dependent transcriptional function (involved in osteoblast differentiation) and ELF and Smad3 involved in the TGF-beta signaling pathway implicated in gastrointestinal cancers.
Biological Activity: Typical enzyme concentration of 100 nM - 5 mM is used for in vitro conjugation, depending on conditions.
Description: Human Praja1 recombinant protein (Genbank Accession No. NP_660095), full length with N-terminal SUMO-tag, expressed in E. coli.
Format: Aqueous buffer solution
Formulation: 50 mM Tris Buffer, pH 7.6, 150 mM NaCl, 10% glycerol
Genbank: NP_660095
Purity: ≥85% by SDS-PAGE
Storage Stability: Stable for≥1 year at -80°C.
Tags: N-terminal SUMO-tag
Uniprot: Q8NG27
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Yu, P., et al. PJA1, encoding a RING-H2 finger ubiquitin ligase, is a novel human X chromosome gene abundantly expressed in brain. Genomics, 2002. 79(6): p. 869-74.
2. Mishra, L., et al. The role of PRAJA and ELF in TGF-beta signaling and gastric cancer. Cancer Biol Ther, 2005. 4(7): p. 694-9.
3. Saha, T., et al. RING finger-dependent ubiquitination by PRAJA is dependent on TGF-beta and potentially defines the functional status of the tumor suppressor ELF. Oncogene, 2006. 25(5): p. 693-705.
4. Sasaki, A., et al., A RING finger protein Praja1 regulates Dlx5-dependent transcription through its ubiquitin ligase activity for the Dlx/Msx-interacting MAGE/Necdin family protein, Dlxin-1. J Biol. Chem, 2002. 277(25): p. 22541-6.
Encompassing Amino Acids: full length
Applications: Useful as an E3 ligase in ubiquitin-conjugating reactions and to analyze upregulation/downregulation of E3 enzymes.
Background: Praja1 (along with Praja2, 53% identity) is a RING-H2 domain E3 (ubiquitin protein ligase) that is involved in the conjugation of ubiquitin to target substrates along with E1 and E2 enzymes. Substrates for Praja1 include Dlxin-1 (MAGE-D1, human) which regulates Dlx5 dependent transcriptional function (involved in osteoblast differentiation) and ELF and Smad3 involved in the TGF-beta signaling pathway implicated in gastrointestinal cancers.
Biological Activity: Typical enzyme concentration of 100 nM - 5 mM is used for in vitro conjugation, depending on conditions.
Description: Human Praja1 recombinant protein (Genbank Accession No. NP_660095), full length with N-terminal SUMO-tag, expressed in E. coli.
Format: Aqueous buffer solution
Formulation: 50 mM Tris Buffer, pH 7.6, 150 mM NaCl, 10% glycerol
Genbank: NP_660095
Purity: ≥85% by SDS-PAGE
Storage Stability: Stable for≥1 year at -80°C.
Tags: N-terminal SUMO-tag
Uniprot: Q8NG27
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Yu, P., et al. PJA1, encoding a RING-H2 finger ubiquitin ligase, is a novel human X chromosome gene abundantly expressed in brain. Genomics, 2002. 79(6): p. 869-74.
2. Mishra, L., et al. The role of PRAJA and ELF in TGF-beta signaling and gastric cancer. Cancer Biol Ther, 2005. 4(7): p. 694-9.
3. Saha, T., et al. RING finger-dependent ubiquitination by PRAJA is dependent on TGF-beta and potentially defines the functional status of the tumor suppressor ELF. Oncogene, 2006. 25(5): p. 693-705.
4. Sasaki, A., et al., A RING finger protein Praja1 regulates Dlx5-dependent transcription through its ubiquitin ligase activity for the Dlx/Msx-interacting MAGE/Necdin family protein, Dlxin-1. J Biol. Chem, 2002. 277(25): p. 22541-6.
| Artikelnummer | BPS81055 |
|---|---|
| Hersteller | BPS Bioscience |
| Hersteller Artikelnummer | 81055 |
| Green Labware | Nein |
| Verpackungseinheit | 25 µg |
| Mengeneinheit | STK |
| Produktinformation (PDF) |
|
| MSDS (PDF) |
|
Immer für Sie da
Gernot Ensinger, M.Sc.
Laurent Haze
