PRKG2, GST-tag Recombinant

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Encompassing Amino Acids: full length

Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 

Background: PRKG2 is a member of the cGMP-dependent protein kinase family and is highly expressed in brain, lung, and intestinal mucosa. PRKG2 mediates intestinal secretion of water and electrolytes induced by the E. coli toxin STa and the intestinal peptide guanylin (1). Mice deficient in PRKG2 are resistant to E. coli STa and developed dwarfism which is caused by a severe defect in endochondral ossification at the growth plates. PRKG2 phosphorylates SOX9 and attenuates SOX9 function by inhibiting its nuclear entry. PRKG2 is a molecular switch that couples the cessation of proliferation and the start of hypertrophic chondrocyte differentiation through attenuating SOX9 function (2).

Description: Recombinant human PRKG2, full length, with N-terminal GST-tag, expressed in Sf9 insect cells via a baculovirus expression system.

Format: Aqueous buffer solution

Formulation: 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol.

Genbank: NM_006259

Host Cell Line: Sf9 cells

Purity: ≥95%

Storage Stability: At least 6 months at -80°C.

Tags: N-terminal GST-tag

Uniprot: Q13237

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: 1. Pfeifer, A. et al: Intestinal secretory defects and dwarfism in mice lacking cGMP-dependent protein kinase II. Science 274: 2082-2084, 1996.
2. Chikuda, H. Et al: Cyclic GMP-dependent protein kinase II is a molecular switch from proliferation to hypertrophic differentiation of chondrocytes. Genes Dev. 18: 2418-2429, 2004.

• Artikelnummer: BPS40170
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 40170
• Green Labware: Nein
• Verpackungseinheit: 10 µg
• Mengeneinheit: STK
• Wirt: Insect