English
PRMT1, His-GST-tag (Sf9-derived) Recombinant
PRMT1, His-GST-tag (Sf9-derived) Recombinant
Artikelnummer
BPS51041
Verpackungseinheit
20 µg
Hersteller
BPS Bioscience
Verfügbarkeit:
wird geladen...
Preis wird geladen...
Products from BPS Bioscience require a minimum order value above 400€
Encompassing Amino Acids: 2-end
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Enzyme was incubated with 0.25 µM of biotinylated H4 1-21 peptide with 1 µM AdoMet in HMT buffer 2A in a 10 µl reaction. 5 µl of Anti-Rabbit AlphaLISA acceptor beads (1:250) and 5 µl of Primary Antibody 4 (1:20) were added and incubation continued for 30 min. Streptavidin Donor beads (1:125) were added (10 µl) and mixture was further incubated for 15 min at RT before A-counts were measured.
Background: PRMT1 is a protein arginine methyltransferase that functions as a histone methyltransferase specific for histone H4. PRMT1 specifically methylates arginine-3 of histone H4 in vitro and in vivo. Methylation of arg3 by PRMT1 facilitates subsequent acetylation of histone H4 tails by p300. PRMT1 performs methylation of arg31 of STAT1 and this is required for transcription induced by IFN-alpha/IFN-beta. Arginine methylation of STAT1 is an additional posttranslational modification regulating transcription factor function, and alteration of arginine methylation might be responsible for the lack of interferon responsiveness observed in many malignancies. Type I PRMTs ((CARM1, PRMT1, PRMT2, PRMT3, PRMT6, and PRMT8) catalyze asymmetric dimethylarginine (ADMA), while type II PRMTs (e.g. PRMTs 5 and 7) catalyze SDMA. PRMT1 is the primary methyltransferase that deposits the ADMA mark, and it accounts for over 90% of this type of methylation.
Description: Human PRMT1 (GenBank Accession No. NM_001536), a.a. 2-end, with N-terminal His-GST-tag, MW= 68 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris pH 8.0, 300 mM NaCl, 20% glycerol and 1 mM DTT.
Genbank: NM_001536
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal His-GST tag
Uniprot: Q99873
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Sun X.J., et al., J Biol Chem. 280, 42, 35261-35271, 2005.
2. Rega S. et al., Mol Cell Neurosci. 18, 1, 68-79, 2001.Application Reference(s):
1. A Sensitive Luminescent Assay for the Histone Methyltransferase NSD1 and Other SAM-Dependent Enzymes (2014)
2. Selective inhibitors of bacterial t-RNA-(N(1)G37) methyltransferase (TrmD) that demonstrate novel ordering of the lid domain (2013)
Encompassing Amino Acids: 2-end
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Enzyme was incubated with 0.25 µM of biotinylated H4 1-21 peptide with 1 µM AdoMet in HMT buffer 2A in a 10 µl reaction. 5 µl of Anti-Rabbit AlphaLISA acceptor beads (1:250) and 5 µl of Primary Antibody 4 (1:20) were added and incubation continued for 30 min. Streptavidin Donor beads (1:125) were added (10 µl) and mixture was further incubated for 15 min at RT before A-counts were measured.
Background: PRMT1 is a protein arginine methyltransferase that functions as a histone methyltransferase specific for histone H4. PRMT1 specifically methylates arginine-3 of histone H4 in vitro and in vivo. Methylation of arg3 by PRMT1 facilitates subsequent acetylation of histone H4 tails by p300. PRMT1 performs methylation of arg31 of STAT1 and this is required for transcription induced by IFN-alpha/IFN-beta. Arginine methylation of STAT1 is an additional posttranslational modification regulating transcription factor function, and alteration of arginine methylation might be responsible for the lack of interferon responsiveness observed in many malignancies. Type I PRMTs ((CARM1, PRMT1, PRMT2, PRMT3, PRMT6, and PRMT8) catalyze asymmetric dimethylarginine (ADMA), while type II PRMTs (e.g. PRMTs 5 and 7) catalyze SDMA. PRMT1 is the primary methyltransferase that deposits the ADMA mark, and it accounts for over 90% of this type of methylation.
Description: Human PRMT1 (GenBank Accession No. NM_001536), a.a. 2-end, with N-terminal His-GST-tag, MW= 68 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris pH 8.0, 300 mM NaCl, 20% glycerol and 1 mM DTT.
Genbank: NM_001536
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal His-GST tag
Uniprot: Q99873
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Sun X.J., et al., J Biol Chem. 280, 42, 35261-35271, 2005.
2. Rega S. et al., Mol Cell Neurosci. 18, 1, 68-79, 2001.Application Reference(s):
1. A Sensitive Luminescent Assay for the Histone Methyltransferase NSD1 and Other SAM-Dependent Enzymes (2014)
2. Selective inhibitors of bacterial t-RNA-(N(1)G37) methyltransferase (TrmD) that demonstrate novel ordering of the lid domain (2013)
| Artikelnummer | BPS51041 |
|---|---|
| Hersteller | BPS Bioscience |
| Hersteller Artikelnummer | 51041 |
| Green Labware | Nein |
| Verpackungseinheit | 20 µg |
| Mengeneinheit | STK |
| Wirt | Insect |
| Produktinformation (PDF) | Download |
| MSDS (PDF) |
|
Immer für Sie da
Gernot Ensinger, M.Sc.
Laurent Haze
