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PRMT3, GST-tag Recombinant
PRMT3, GST-tag Recombinant
Artikelnummer
BPS51043
Verpackungseinheit
50 µg
Hersteller
BPS Bioscience
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Encompassing Amino Acids: 2-end
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: 50 µl reaction mix (20 mM phosphate buffer pH 7.4, 20 µM S-adenosyl methionine, and 1-10 ng methyltransferase PRMT3) add to the wells coated with the substrate. Incubate at room temperature for 1 hr. Add antibody against methylated R3 residue of histone H4, incubate 1 hr. Add secondary HRP-labeled antibody and incubate 30 min. Finally, add HRP chemiluminescent substrates and read luminesence.
Background: PRMT3 is a protein arginine methyltransferase that catalyze the formation of asymmetric N(G),N(G)-dimethylarginine (ADMA) residues in proteins. 40S ribosomal protein S2 is a physiological substrate of PRMT3 and PRMT3 regulates ribosome biosynthesis at a stage beyond pre-rRNA processing. DAL-1 (differentially expressed in adenocarcinoma of the lung)/4.1B is a tumor suppressor gene that can significantly inhibits PRMT3 methylation of cellular substrates. Modulation of post-translational methylation may be an important mechanism through which DAL-1/4.1B affects tumor cell growth.
Description: Human PRMT3 (GenBank Accession No. NM_005788), a.a. 2-end, with N-terminal GST-tag, MW=86 kDa, expressed in an E. coli expression system.
Format: Aqueous buffer solution
Formulation: 45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 18 mM glutathione, 3 mM DTT, 10% glycerol
Genbank: NM_005788
Host Cell Line: E. coli
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: Q8WUV3
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Choi S. et al., Biochim Biophys Acta. 2008 Sep,1780, 9, :1062-9.
2. Bachand F. and Silver P.A., EMBO J. 2004 Jul 7,23, 13, :2641-50.
Encompassing Amino Acids: 2-end
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: 50 µl reaction mix (20 mM phosphate buffer pH 7.4, 20 µM S-adenosyl methionine, and 1-10 ng methyltransferase PRMT3) add to the wells coated with the substrate. Incubate at room temperature for 1 hr. Add antibody against methylated R3 residue of histone H4, incubate 1 hr. Add secondary HRP-labeled antibody and incubate 30 min. Finally, add HRP chemiluminescent substrates and read luminesence.
Background: PRMT3 is a protein arginine methyltransferase that catalyze the formation of asymmetric N(G),N(G)-dimethylarginine (ADMA) residues in proteins. 40S ribosomal protein S2 is a physiological substrate of PRMT3 and PRMT3 regulates ribosome biosynthesis at a stage beyond pre-rRNA processing. DAL-1 (differentially expressed in adenocarcinoma of the lung)/4.1B is a tumor suppressor gene that can significantly inhibits PRMT3 methylation of cellular substrates. Modulation of post-translational methylation may be an important mechanism through which DAL-1/4.1B affects tumor cell growth.
Description: Human PRMT3 (GenBank Accession No. NM_005788), a.a. 2-end, with N-terminal GST-tag, MW=86 kDa, expressed in an E. coli expression system.
Format: Aqueous buffer solution
Formulation: 45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 18 mM glutathione, 3 mM DTT, 10% glycerol
Genbank: NM_005788
Host Cell Line: E. coli
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: Q8WUV3
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Choi S. et al., Biochim Biophys Acta. 2008 Sep,1780, 9, :1062-9.
2. Bachand F. and Silver P.A., EMBO J. 2004 Jul 7,23, 13, :2641-50.
| Artikelnummer | BPS51043 |
|---|---|
| Hersteller | BPS Bioscience |
| Hersteller Artikelnummer | 51043 |
| Green Labware | Nein |
| Verpackungseinheit | 50 µg |
| Mengeneinheit | STK |
| Produktinformation (PDF) | Download |
| MSDS (PDF) |
|
Immer für Sie da
Gernot Ensinger, M.Sc.
Laurent Haze
