PRMT4 Chemiluminescent Assay Kit

Products from BPS Bioscience require a minimum order value above 400€

Applications: Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.

Background: PRMT4 (CARM1) is a histone methyltransferase that exhibits methylation activity toward H3-R17.

Contraindications: DMSO >1%, strong acids or bases, ionic detergents, high salt

Description: The PRMT4 Direct Activity Chemiluminescent Assay kit is designed to measure PRMT4/CARM1 activity activity for screening and profiling applications. The key to the PRMT4Chemiluminescent Assay Kit is a highly specific antibody that recognizes methylatedPRMT4 substrate. With this kit, only three simple steps on a microtiter plate are requiredfor methyltransferase detection. First, S-adenosylmethionine is incubated with a samplecontaining assay buffer and methyltransferase enzyme. Next, primary antibody is added.Finally, the strip plates are treated with an HRP-labeled secondary antibody followed byaddition of an HRP substrate to produce chemiluminescence that can then be measuredusing a chemiluminescence reader.

Storage Stability: 12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Supplied As: Kit comes in a convenient format, with 8-well strips precoated with the specific PRMT4 peptide substrate, reagents, and purified PRMT4 enzyme for 96 enzyme reactions.

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: Yang, Y., Bedford, M.T. 2013. Nat Rev Cancer. 13(1):37-50.

• Artikelnummer: BPS52041L
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 52041L
• Green Labware: Nein
• Verpackungseinheit: 96 rxns.
• Mengeneinheit: PAK