PRMT4 Homogeneous Assay Kit

Products from BPS Bioscience require a minimum order value above 400€

Applications: Great for studying enzyme kinetics and HTS applications.

Contraindications: Green and blue dyes that absorb light in the AlphaScreen® signal emission range (520-620 nm), such as Trypan Blue. Avoid the use of the potent singlet oxygen quenchers such as sodium azide (NaN3) or metal ions (Fe2+, Fe3+, Cu2+, Zn2+ and Ni2+). The presence of >1% RPMI 1640 culture medium leads to a signal reduction due to the presence of excess biotin and iron in this medium. MEM, which lacks these components, does not affect AlphaScreen® assays.

Description: The PRMT4 Homogeneous Assay Kit is designed to measure PRMT4 activity for screening and profiling applications. PRMT4 (CARM1) is an arginine methyltransferase and dual functional coregulator that facilitates transcription initiation by methylation of Arg17 and Arg26 of histone H3. The PRMT4 Homogeneous Assay Kit comes in a convenient AlphaLISA® format, with His-tagged PRMT4 substrate, primary antibody, methylation assay buffer, and purified PRMT4 for 384 enzyme reactions. The key to the PRMT4 Homogeneous Assay Kit is a highly specific antibody that recognizes methylated substrate. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, a sample containing PRMT4 enzyme is incubated with the substrate. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts.

Storage Stability: 12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Supplied As: Kit comes in a convenient AlphaLISA® format, with biotinylated histone H4 peptide substrate, primary antibody, methylation assay buffer, and purified PRMT5 for 384 enzyme reactions.

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: Yang, Y., Bedford, M.T. 2013. Nat Rev Cancer. 13(1):37-50.

• Artikelnummer: BPS52068
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 52068
• Green Labware: Nein
• Verpackungseinheit: 384 rxns.
• Mengeneinheit: PAK