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PRMT5/MEP50, Biotin-Labeled Recombinant
PRMT5/MEP50, Biotin-Labeled Recombinant
Artikelnummer
BPS52102
Verpackungseinheit
10 µg
Hersteller
BPS Bioscience
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Encompassing Amino Acids: 2-end (both)
Background: Three types of arginine methylation exist in mammalian cells: monomethylarginine (MMA), asymmetric dimethylarginine (ADMA) and symmetric dimethylarginine (SDMA). Type I PRMTs ((CARM1, PRMT1, PRMT2, PRMT3, PRMT6, and PRMT8) catalyze ADMA, while type II PRMTs (e.g. PRMTs 5 and 7) catalyze SDMA. Type II PRMTs are found to be strongly implicated in diseases like cancer. For example, PRMT5 plays a role in the repression of certain tumor suppressor genes such as RB tumor suppressors while PRMT7 overexpression is observed in breast cancer
Description: PRMT5/MEP50 complex of human PRMT5 (GenBank Accession No. NM_006109), amino-acids 2-637(end), with N-terminal FLAG-Avi tag, MW=73 kDa, and human MEP50 (GenBank Accession No. NM_024102) with N-terminal His-Tag, amino-acids 2-342(end), MW=38 kDa, coexpressed in HEK293 cells and enzymatically biotin-labeled using Avi-tag™ technology.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl pH 8.0, 110 mM NaCl, 2.2 mM KCl, 80 µg/mL FLAG peptide, 20% glycerol and 3 mM DTT.
Genbank: NM_006109 (PRMT5), NM_024102 (MEP50)
Host Cell Line: HEK293
Storage Stability: At least 6 months at -80°C. Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended, if necessary, use carrier protein (BSA 0.1 - 0.5%).
Tags: N-terminal FLAG-Avi-tag (PRMT5), N-terminal His-tag (MEP50)
Uniprot: PRMT5: O14744; MEP50: Q9BQA1
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Zhao Q. et al., J. Nat. Struct. Mol. Biol. 16 (3), 304-311 (2009).
2. Wang L et al., Mol. Cell. Biol. 28 (20), 6262-6277 (2008).
Encompassing Amino Acids: 2-end (both)
Background: Three types of arginine methylation exist in mammalian cells: monomethylarginine (MMA), asymmetric dimethylarginine (ADMA) and symmetric dimethylarginine (SDMA). Type I PRMTs ((CARM1, PRMT1, PRMT2, PRMT3, PRMT6, and PRMT8) catalyze ADMA, while type II PRMTs (e.g. PRMTs 5 and 7) catalyze SDMA. Type II PRMTs are found to be strongly implicated in diseases like cancer. For example, PRMT5 plays a role in the repression of certain tumor suppressor genes such as RB tumor suppressors while PRMT7 overexpression is observed in breast cancer
Description: PRMT5/MEP50 complex of human PRMT5 (GenBank Accession No. NM_006109), amino-acids 2-637(end), with N-terminal FLAG-Avi tag, MW=73 kDa, and human MEP50 (GenBank Accession No. NM_024102) with N-terminal His-Tag, amino-acids 2-342(end), MW=38 kDa, coexpressed in HEK293 cells and enzymatically biotin-labeled using Avi-tag™ technology.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl pH 8.0, 110 mM NaCl, 2.2 mM KCl, 80 µg/mL FLAG peptide, 20% glycerol and 3 mM DTT.
Genbank: NM_006109 (PRMT5), NM_024102 (MEP50)
Host Cell Line: HEK293
Storage Stability: At least 6 months at -80°C. Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended, if necessary, use carrier protein (BSA 0.1 - 0.5%).
Tags: N-terminal FLAG-Avi-tag (PRMT5), N-terminal His-tag (MEP50)
Uniprot: PRMT5: O14744; MEP50: Q9BQA1
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Zhao Q. et al., J. Nat. Struct. Mol. Biol. 16 (3), 304-311 (2009).
2. Wang L et al., Mol. Cell. Biol. 28 (20), 6262-6277 (2008).
| Artikelnummer | BPS52102 |
|---|---|
| Hersteller | BPS Bioscience |
| Hersteller Artikelnummer | 52102 |
| Green Labware | Nein |
| Verpackungseinheit | 10 µg |
| Mengeneinheit | STK |
| Wirt | Human |
| Produktinformation (PDF) | Download |
| MSDS (PDF) |
|
Immer für Sie da
Gernot Ensinger, M.Sc.
Laurent Haze
