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PRMT7 Homogeneous Assay Kit (Automethylation)
PRMT7 Homogeneous Assay Kit (Automethylation)
Artikelnummer
BPS79721
Verpackungseinheit
384 rxns.
Hersteller
BPS Bioscience
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Products from BPS Bioscience require a minimum order value above 400€
Applications: Great for studying enzyme kinetics and HTS applications.
Contraindications: Green and blue dyes that absorb light in the AlphaScreen® signal emission range (520-620 nm), such as Trypan Blue. Avoid the use of the potent singlet oxygen quenchers such as sodium azide (NaN3) or metal ions (Fe2+, Fe3+, Cu2+, Zn2+ and Ni2+). The presence of >1% RPMI 1640 culture medium leads to a signal reduction due to the presence of excess biotin and iron in this medium. MEM, which lacks these components, does not affect AlphaScreen® assays.
Description: The PRMT7 Homogeneous Assay Kit (Automethylation) is designed to measure PRMT7 automethylation for screening and profiling applications. Automethylation of PRMT7 plays a key role in inducing the epithelial-mesenchymal transition program and in promoting the migratory and invasive behavior of breast cancer cells. The key to the PRMT7 Homogeneous Assay Kit (Automethylation) is a highly specific antibody that recognizes methylated PRMT7. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, a sample containing PRMT7 enzyme is incubated with SAM. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts.Note: To study methylation of histone proteins by PRMT7, we recommend using our PRMT7 Homogeneous Assay Kit (H2b), #79720.
Storage Stability: At least one year from date of receipt when stored as directed.
Supplied As: The PRMT7 Homogeneous Assay Kit (Automethylation) comes in a convenient AlphaLISA® format, with S-adenosylmethionine (SAM), primary antibody, methylation assay buffer, and purified Flag-tagged PRMT7 for 384 enzyme reactions.
Warnings: Avoid multiple freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. You Feng, et al. 2014. J Biol Chem 289:32604.
2. Pengyu Geng, et al. 2017. FASEB J. 31:2287.
Applications: Great for studying enzyme kinetics and HTS applications.
Contraindications: Green and blue dyes that absorb light in the AlphaScreen® signal emission range (520-620 nm), such as Trypan Blue. Avoid the use of the potent singlet oxygen quenchers such as sodium azide (NaN3) or metal ions (Fe2+, Fe3+, Cu2+, Zn2+ and Ni2+). The presence of >1% RPMI 1640 culture medium leads to a signal reduction due to the presence of excess biotin and iron in this medium. MEM, which lacks these components, does not affect AlphaScreen® assays.
Description: The PRMT7 Homogeneous Assay Kit (Automethylation) is designed to measure PRMT7 automethylation for screening and profiling applications. Automethylation of PRMT7 plays a key role in inducing the epithelial-mesenchymal transition program and in promoting the migratory and invasive behavior of breast cancer cells. The key to the PRMT7 Homogeneous Assay Kit (Automethylation) is a highly specific antibody that recognizes methylated PRMT7. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, a sample containing PRMT7 enzyme is incubated with SAM. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts.Note: To study methylation of histone proteins by PRMT7, we recommend using our PRMT7 Homogeneous Assay Kit (H2b), #79720.
Storage Stability: At least one year from date of receipt when stored as directed.
Supplied As: The PRMT7 Homogeneous Assay Kit (Automethylation) comes in a convenient AlphaLISA® format, with S-adenosylmethionine (SAM), primary antibody, methylation assay buffer, and purified Flag-tagged PRMT7 for 384 enzyme reactions.
Warnings: Avoid multiple freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. You Feng, et al. 2014. J Biol Chem 289:32604.
2. Pengyu Geng, et al. 2017. FASEB J. 31:2287.
| Artikelnummer | BPS79721 |
|---|---|
| Hersteller | BPS Bioscience |
| Hersteller Artikelnummer | 79721 |
| Green Labware | Nein |
| Verpackungseinheit | 384 rxns. |
| Mengeneinheit | PAK |
| Produktinformation (PDF) | Download |
| MSDS (PDF) |
|
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