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PTPµ (PTPRM), GST-tag Recombinant
PTPµ (PTPRM), GST-tag Recombinant
Artikelnummer
BPS30053
Verpackungseinheit
50 µg
Hersteller
BPS Bioscience
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Encompassing Amino Acids: 896-1175
Applications: Useful for the study of enzyme kinetics and regulation, to dephosphorylate target substrates and for screening inhibitors.
Assay Conditions: 20 mM HEPES, pH 7.2, 100 mM NaCl, 2 mM EDTA, 1 mM DTT, 1 mM pNPP and 2 ng/µl PTP-mu. Assay was done at room temperature for 5 minutes, and the reaction was monitored at 405 nm continuously. Specific activity was calculated based on ε pNPP= 18,000 M-1cm-1.
Background: PTPRM is a member of the protein tyrosine phosphatase family and can participate in a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. PTPRM has been shown to mediate cell-cell aggregation through the interaction with another molecule of PTPRM on an adjacent cell. PTPRM can interact with scaffolding protein RACK1/GNB2L1 and this interaction may be necessary for downstream signaling in response to cell-cell adhesion. PTPRM has been shown to be expressed in human pulmonary vascular endothelia where it directly binds to VE-cadherin and regulates both the tyrosine phosphorylation state of VE-cadherin and barrier integrity.
Description: PTPµ (mu) (protein tyrosinephosphatase mu, also known as RPTPµ),catalytic domain, (a.a. 896-1175), (GenBankAccession No. NM_001105244) withN-terminal GST tag, MW=56.2 kDa,expressed in an E. coli expression system.
Format: Aqueous buffer solution
Formulation: 25 mM Tris-HCl, pH 8.0, 75 mM NaCl, 0.05% Tween-20, 50% glycerol, 2 mM EDTA, 1 mM DTT, 10 mM glutathione
Genbank: NM_001105244
Storage Stability: >12 months at -80°C
Tags: N-terminal GST-tag
Uniprot: P28827
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. S. Brady-Kalnay, et al., J. Cell Biol 1995, 130: 977.
2. T. Mourton, et al., J. Biol. Chem. 2002, 276:14896.
Encompassing Amino Acids: 896-1175
Applications: Useful for the study of enzyme kinetics and regulation, to dephosphorylate target substrates and for screening inhibitors.
Assay Conditions: 20 mM HEPES, pH 7.2, 100 mM NaCl, 2 mM EDTA, 1 mM DTT, 1 mM pNPP and 2 ng/µl PTP-mu. Assay was done at room temperature for 5 minutes, and the reaction was monitored at 405 nm continuously. Specific activity was calculated based on ε pNPP= 18,000 M-1cm-1.
Background: PTPRM is a member of the protein tyrosine phosphatase family and can participate in a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. PTPRM has been shown to mediate cell-cell aggregation through the interaction with another molecule of PTPRM on an adjacent cell. PTPRM can interact with scaffolding protein RACK1/GNB2L1 and this interaction may be necessary for downstream signaling in response to cell-cell adhesion. PTPRM has been shown to be expressed in human pulmonary vascular endothelia where it directly binds to VE-cadherin and regulates both the tyrosine phosphorylation state of VE-cadherin and barrier integrity.
Description: PTPµ (mu) (protein tyrosinephosphatase mu, also known as RPTPµ),catalytic domain, (a.a. 896-1175), (GenBankAccession No. NM_001105244) withN-terminal GST tag, MW=56.2 kDa,expressed in an E. coli expression system.
Format: Aqueous buffer solution
Formulation: 25 mM Tris-HCl, pH 8.0, 75 mM NaCl, 0.05% Tween-20, 50% glycerol, 2 mM EDTA, 1 mM DTT, 10 mM glutathione
Genbank: NM_001105244
Storage Stability: >12 months at -80°C
Tags: N-terminal GST-tag
Uniprot: P28827
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. S. Brady-Kalnay, et al., J. Cell Biol 1995, 130: 977.
2. T. Mourton, et al., J. Biol. Chem. 2002, 276:14896.
| Artikelnummer | BPS30053 |
|---|---|
| Hersteller | BPS Bioscience |
| Hersteller Artikelnummer | 30053 |
| Green Labware | Nein |
| Verpackungseinheit | 50 µg |
| Mengeneinheit | STK |
| Produktinformation (PDF) | Download |
| MSDS (PDF) |
|
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Gernot Ensinger, M.Sc.
Laurent Haze
