PYK2, His-tag Recombinant

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Encompassing Amino Acids: full length

Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 

Assay Conditions: ADP-Glo assay with 0.1 mg/ml poly-(Glu4:Tyr) as a substrate and 100 mM ATP at final concentration. Reaction was done for 60 minutes at 30°C. ADP-Glo reagents were added for 45 minutes at RT, and Kinase detection reagents for 55 minutes at RT, followed by luminescence detection.

Description: Human PYK2 (GenBank Accession No. U33284), full length with N-terminal His-tag, MW=120 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Format: Aqueous buffer solution

Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 200 mM imidazole

Genbank: U33284

Host Cell Line: Sf9 cells

Storage Stability: At least 6 months at -80°C.

Tags: N-terminal His-tag

Uniprot: Q14289

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: 1. Cain R.J. et al., J Cell Biol. 2010 Mar 22,188(6):863-76.
2. Collins M. et al., Mol Immunol. 2010 May,47(9):1665-74.

• Artikelnummer: BPS40480
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 40480
• Green Labware: Nein
• Verpackungseinheit: 10 µg
• Mengeneinheit: STK
• Wirt: Insect