Pyranose Oxidase Antibody

Application Note: Anti-Pyranose Oxidase has been tested by western blot and is suitable to be assayed against 1.0 ug of Pyranose Oxidase [E.coli] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Rabbit) code #605-4302 and (ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:50,000 to 1:400,000 of the reconstitution concentration is suggested for this product.

Concentration Value: 90 mg/mL

ELISA Dilution: 1:5,000 - 1:20,000

Immunoprecipitation Dilution: 1:100

Western Blot Dilution: 1:500 - 1:5,000

General Disclaimer Note: This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen: Pyranose Oxidase [E.coli]

Physical State: Lyophilized

Purity and Specificity: This product was prepared from monospecific antiserum by a delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, purified and partially purified Pyranose Oxidase [E.coli].  Cross reactivity against Pyranose Oxidase from other sources is unknown.

Background: Pyranose Oxidase catalyzes the oxidation of various aldopyranoses and disaccharides on carbon-2 to the corresponding 2-keto sugars concomitant with the reduction of O2 to H2O2. It plays an important role in lignin degradation of wood rot fungi by supplying the essential cosubstrate H2O2 for the ligninolytic peroxidases, lignin peroxidase, and manganese-dependent peroxidase. The preferred substrate is D-glucose which is converted to 2-dehydro-D-glucose. Acts also on D-xylose, together with D-glucose the major sugars derived from wood, on L-sorbose, D-galactose and 1,5-anhydroglucitol, a diagnostic marker of diabetes mellitus.

Low Endotoxin: No

Other: User Optimized

• Artikelnummer: ROC100-101-235
• Hersteller: Rockland
• Hersteller Artikelnummer: 100-101-235
• Verpackungseinheit: 2 mL
• Mengeneinheit: STK
• Reaktivität: Various species
• Klonalität: Polyclonal
• Methode: Western Blotting
• Isotyp: Antiserum
• Wirt: Goat
• Konjugat: Unconjugated