Usage: This ELISA kit applies to the in vitro quantitative determination of Rat IL-1R2 concentrations in serum, plasma.
Tested Sample Types: serum, plasma
Assay Time: 1.5h
Sensitivity: 18.75 pg/mL
Detection Range: 31.25-2000 pg/mL
UNIProt ID: P43303
Test Principle: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat IL-1R2. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Rat IL-1R2 are added to the micro ELISA plate wells. Rat IL-1R2 in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Rat IL-1R2.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The OD value is proportional to the concentration of Rat IL-1R2. The concentration of Rat IL-1R2 in the samples is then determined by comparing the OD of the samples to the standard curve.
Cross reactivity: This kit recognizes Rat IL-1R2 in samples.No significant cross-reactivity or interference between Rat IL-1R2 and analogues was observed