Rab4 Antibody: HRP

Rabbit Anti-Human Rab4 Polyclonal

Target: Rab4

Conjugate: HRP

Product Type: Polyclonal

Immunogen: C-terminal peptide from human Rab4

Swiss-Prot: P20338

Purification: Peptide Affinity Purified

Storage Buffer: PBS pH7.4, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated

Concentration: 1 mg/ml

Specificity: Detects ~26kDa.

Cellular Localization: Membrane,Cytoplasm

Scientific Background: Rab4 is a 25kDa member of the Rab family of small guanosine triphosphatases (GTPases), Ras superfamily. Rab GTPases are central regulators of membrane trafficking in the eukaryotic cell. Their regulatory capacity depends on their ability to cycle between the GDP -bound inactive and GTP-bound active states. This conversion is regulated by GDP/GTP exchange factors (GEPs), GDP dissociation inhibitors (GDIs) and GTPase-activating proteins (GAPs) (1, 2). Activation of a Rab protein is coupled to its association with intracellular membranes, allowing it to recruit downstream effector proteins to the cytoplasmic surface of a sub-cellular compartment (3). Through these proteins, Rab GTPases regulate vesicle formation, actin- and tubulin-dependent vesicle movement, and membrane fusion(1). Rab proteins contain conserved regions involved in guanine-nucleotide binding, and hyper-variable COHO-terminal domains with a cysteine motif implicated in sub-cellular targeting. Post-translational modification of the cysteine motif with one or two geranylgeranyl groups is essential for the membrane association and correct intracellular localization of Rab proteins (3). Each Rab shows a characteristic sub-cellular distribution (4).In particular, over-expression of Rab4 causes a redistribution of receptors on plasma membrane versus endocytic compartments. The presence of excessive Rab4 leads to the accumulation of tranferrin receptors in non-acidic, post-endosomal recycling vesicles considered an intermediate compartment between endosomes and plasma membranes. Rab4 also plays a role in the translocation of glucose transporter (Glu4) in adipocytes in response to insulin (5). Mediating the association of Rab4 with transferring receptor-containing early endosomes takes place through the geranylgeranyl groups at its carboxyl-terminus. Membrane association is also cell cycle dependent, as phosphorylation at its c-terminal cdc2 kinase consensus sequence in mitotic cells leads to dissociation of Rab4 into the cytosol (6).

References: 1. Stenmark H., and Olkkonen V.M. (2001) Genome Biol. 2: 3007.1-3007.7.2. Takai Y., et al. (2001) Physiol. Rev. 8:, 153-208.3. Ali B.R., et al. (2004) J. Cell Sci. 117: 6401-6412.4. Zerial M., and McBride H. (2001) Nat. Rev. Mol. Cell Biol. 2: 107-117.5. Cormont M., et al. (1996) Mol Cell Biology. 16: 6879-6886.6. Ayad N., Hull M., and Mellman I. (1997) EMBO 16: 4497-4507.7. Van der Sluijs, P. et l. (1992) The EMBO Journal 11(12), 4379-4389.

Field of Use: Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

• Artikelnummer: STRSPC-141D-HRP
• Hersteller: Stressmarq Biosciences
• Hersteller Artikelnummer: SPC-141D-HRP
• Green Labware: Nein
• Verpackungseinheit: 100 µl
• Mengeneinheit: STK
• Reaktivität: Human, Mouse (Murine), Rat (Rattus)
• Klonalität: Polyclonal
• Methode: Immunofluorescence, Western Blotting, Immunohistochemistry, Immunocytochemistry
• Human Gene ID: 5867
• Wirt: Rabbit