Rabbit anti Human Beta-secretase 2 (CT)

Rabbit anti Human Beta-secretase 2 (CT), Polyclonal, IgG

Background: Accumulation of the amyloid- (A ) plaque in the cerebral cortex is a critical event in the pathogenesis of AlzheimerÕs disease. A peptide is generated by proteolytic cleavage of the amyloid protein precursor (APP) at - and -sites by proteases. The long-sought secretase was recently identified by several groups independently and designated beta-site APP cleaving enzyme (BACE) and aspartyl protease 2 (Asp2)1-4. A BACE homolog was recently cloned and designated BACE2, Asp1, DRAP (for Down region aspartic protease), and memapsin 14-9. BACE2 also cleaves APP at -site and at a different site within A 8. BACE2 locates on chromosome 21q22.3, the so-called ÔDown critical regionÕ, suggesting that BACE2 and A may also contribute to the pathogenesis of Down syndrome6,7.

Positive Control: Human heart tissue lysate.

Immunogen: Synthetic peptide corresponding to amino acids 496 to 511 of human BACE2.

Purification Method: Antigen Immunoaffiinity Purification.

Formulation: Provided in phosphate buffered saline solution containing 0.02% sodium azide as a preservative.

References: 1. Vassar, R., et al. Beta-secretase cleavage of Alzheimer's amyloid precursor protein by the transmembrane aspartic protease BACE. Science 1999, 286, 735-741
2. Hussain, I., et al. Identification of a novel aspartic protease (Asp 2) as beta-secretase. Mol. Cell Neurosci. 1999, 14, 419-427
3. Yan, R., et al. Membrane-anchored aspartyl protease with Alzheimer's disease beta-secretase activity. Nature 1999, 402, 533-537
4. Sinha, S., et al. Purification and cloning of amyloid precursor protein beta-secretase from human brain. Nature 1999, 402, 537-540
5. Lin, X., et al. Human aspartic protease memapsin 2 cleaves the beta-secretase site of beta-amyloid precursor protein. Proc. Natl. Acad. Sci. USA 2000 15, 97, 1456-1460
6. Acquati, F., et al. The gene encoding DRAP (BACE2), a glycosylated transmembrane protein of the aspartic protease family, maps to the down critical region. FEBS Lett. 2000, 468, 59-64
7. Solans, A., et al. A new aspartyl protease on 21q22.3, BACE2, is highly similar to Alzheimer's amyloid precursor protein beta-secretase. Cytogenet. Cell Genet. 2000, 89, 177-184
8. Farzan, M., et al. BACE2, a beta -secretase homolog, cleaves at the beta site and within the amyloid-beta region of the amyloid-beta precursor protein. Proc. Natl. Acad. Sci. USA 2000, 97, 9712-9717
9. Bennett, B.D., et al. Expression analysis of BACE2 in brain and peripheral tissues. J. Biol. Chem. 2000, 275, 20647-20651.

UniProt: Q9Y5Z0.

Caution: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.

• Artikelnummer: EXAX1137P
• Hersteller: Exalpha Biologicals Inc
• Hersteller Artikelnummer: X1137P
• Green Labware: Nein
• Verpackungseinheit: 100 µg
• Mengeneinheit: STK
• Reaktivität: Human
• Klonalität: Polyclonal
• Methode: Western Blotting
• Isotyp: IgG
• Wirt: Rabbit