Rabbit anti Human CCR5 (NT) (HIV and chemokine receptor)

Rabbit anti Human CCR5 (NT) (HIV and chemokine receptor), Polyclonal, IgG

Background: Human immunodeficiency virus (HIV) and related virus require coreceptors, in addition to CD4, to infect target cells. Some G protein-coupled receptors including CCR5, CXCR4, CCR3, CCR2b and CCR8 in the chemokine receptor family, and four new human molecules GPR15, STRL33, GPR1 and V28 were recently identified as HIV coreceptors1,2. Among them, CCR5 (CC-CKR-5) is a principal coreceptor for macrophage- and dual-tropic HIV-1 strains fusion and entry of human white blood cells3,4. CCR5 is required for the infection by HIV-1, HIV-2, and SIV1,2. The -chemokines RANTES, MIP-1 and MIP-1 are the ligands for CCR5 and prevent infection by M-tropic HIV-13-5. CXC5 associates with the surface CD4-gp120 of HIV complex and leads to membrane fusion and virus entry of target cells6,7. The amino-terminal domain and the extracellular loops of CCR5 serve as HIV biding sites8. CCR5 messenger RNA is expressed in lymphoid organs and monocytes.

Positive Control: THP-1 whole cell lysates.

Immunogen: Synthetic peptide corresponding to amino acids 6 to 20 of human CCR5.

Purification Method: Antigen Immunoaffiinity Purification.

Formulation: Provided in phosphate buffered saline solution containing 0.02% sodium azide as a preservative.

References: 1. Dimitrov, D.S. How do viruses enter cells? The HIV coreceptors teach us a lesson of complexity. Cell 1997, 91, 721-730

2. Littman, D.R. Chemokine receptors: keys to AIDS pathogenesis? Cell 1998, 93, 677-680

3. Deng, H., et al. Identification of a major co-receptor for primary isolates of HIV-1. Nature 1996, 381, 661-666

4. Dragic, T., et al. HIV-1 entry into CD4+ cells is mediated by the chemokine receptor CC-CKR-5. Nature 1996, 381, 667-673

5. Coochi, F. et al. Identification of RANTES, MIP-1 alpha, and MIP-1 beta as the major HIV-suppressive factors produced by CD8+ T cells. Science 1995, 270, 1811-1815.

6. Wu, L., et al. CD4-induced interaction of primary HIV-1 gp120 glycoproteins with the chemokine receptor CCR-5. Nature 1996, 384, 179-183

7. Trkola , A. et al. CD4-dependent, antibody-sensitive interactions between HIV-1 and its co-receptor CCR-5. Nature 1996, 384, 184-187

8. Doranz, B.J. et al. Two distinct CCR5 domains can mediate coreceptor usage by human immunodeficiency virus type 1. J. Virol. 1997, 71, 6305-6314.

UniProt: P51681.

Caution: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.

• Artikelnummer: EXAX1144P
• Hersteller: Exalpha Biologicals Inc
• Hersteller Artikelnummer: X1144P
• Green Labware: Nein
• Verpackungseinheit: 100 µg
• Mengeneinheit: STK
• Reaktivität: Human
• Klonalität: Polyclonal
• Methode: Western Blotting
• Isotyp: IgG
• Wirt: Rabbit