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Encompassing Amino Acids: 30-212
Applications: Useful for studying protein binding and for screening small molecules and antibodies.
Description: Human Receptor Activator of Nuclear Factor κB (RANK), also known as TRANCE Receptor and TNFRSF11A, (Genbank Accession No. NM_003839), a.a. 30-212, fused at the C-terminus to the Fc portion of human IgG1, with a C-terminal Avi-tag, expressed in a HEK293 cell expression system. MW = 49 kDa. This protein runs at a higher MW by SDS-PAGE due to glycosylation
Format: Aqueous buffer solution
Formulation: 8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol.
Genbank: NM_003839
Storage Stability: At least 6 months at -80°C. Avoid freeze/thaw cycles. Protein may be diluted to ≥ 100 µg/ml in PBS + glycerol and stored at -80°C.
Tags: C-terminal Fc fusion (Human IgG1), C-terminal Avi-tag™
Uniprot: Q9Y6Q6
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1.Annis, A., J. American Chemical Society., 2004 May; 126(4): 15495-15503.2.Yan, T., J. Cellular Biochemistry. 2001 Aug ; 83(2):320-325.