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Rat PTP1B(1-321)/PTPN1, GST-tag Recombinant
Rat PTP1B(1-321)/PTPN1, GST-tag Recombinant
Artikelnummer
BPS30011
Verpackungseinheit
50 µg
Hersteller
BPS Bioscience
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Encompassing Amino Acids: 1-321
Applications: Useful for the study of enzyme kinetics and regulation, to dephosphorylate target substrates and for screening inhibitors.
Assay Conditions: The activity of rat PTP1B was determined using pNPP. Assay conditions: 50 mM HEPES, pH 7.4, 2 mM EDTA, 3 mM DTT, 100 mM NaCl, 20 mM pNPP, 5 minute incubation at 30°C, using 2 µg/ml enzyme.
Background: Protein tyrosine phosphatase nonreceptor, type 1 (PTPN1) is the founding member of the protein tyrosine phosphatase (PTP) family. PTPN1 catalyze the hydrolysis of the phosphate monoesters specifically from tyrosine residues. PTPN1 is known to be involved in signaling pathways that regulate a variety of cellular processes including cell growth and oncogenic transformation. PTPN1 also has been shown to act as a negative regulator of insulin signaling. PTPN1 has also been reported to dephosphorylate epidermal growth factor receptor kinase, which implicated the role of this phosphatase in cell growth control and cell response to interferon stimulation.
Description: Rat recombinant PTP1B Protein tyrosine phosphatase, also known as PTPN1 (Genbank accession No. NM_012637.2), a.a. 1-321 (catalytic domain), with N-terminal GST-tag, MW=63.3 kDa, expressed in an E. coli expression system.
Format: Aqueous buffer solution
Formulation: 25 mM Tris-HCl, 75 mM NaCl, pH 8.0, 0.05% Tween-20, 10 mM glutathione, 1 mM DTT and 50% glycerol
Genbank: NM_012637.2
Storage Stability: >12 months at -80°C
Tags: N-terminal GST-tag
Unit Definition: One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30°C.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Frangioni et al., Cell, 1992, 68: 545.
2. M. Elchebly et al., Science 1999, 283: 1544.
Encompassing Amino Acids: 1-321
Applications: Useful for the study of enzyme kinetics and regulation, to dephosphorylate target substrates and for screening inhibitors.
Assay Conditions: The activity of rat PTP1B was determined using pNPP. Assay conditions: 50 mM HEPES, pH 7.4, 2 mM EDTA, 3 mM DTT, 100 mM NaCl, 20 mM pNPP, 5 minute incubation at 30°C, using 2 µg/ml enzyme.
Background: Protein tyrosine phosphatase nonreceptor, type 1 (PTPN1) is the founding member of the protein tyrosine phosphatase (PTP) family. PTPN1 catalyze the hydrolysis of the phosphate monoesters specifically from tyrosine residues. PTPN1 is known to be involved in signaling pathways that regulate a variety of cellular processes including cell growth and oncogenic transformation. PTPN1 also has been shown to act as a negative regulator of insulin signaling. PTPN1 has also been reported to dephosphorylate epidermal growth factor receptor kinase, which implicated the role of this phosphatase in cell growth control and cell response to interferon stimulation.
Description: Rat recombinant PTP1B Protein tyrosine phosphatase, also known as PTPN1 (Genbank accession No. NM_012637.2), a.a. 1-321 (catalytic domain), with N-terminal GST-tag, MW=63.3 kDa, expressed in an E. coli expression system.
Format: Aqueous buffer solution
Formulation: 25 mM Tris-HCl, 75 mM NaCl, pH 8.0, 0.05% Tween-20, 10 mM glutathione, 1 mM DTT and 50% glycerol
Genbank: NM_012637.2
Storage Stability: >12 months at -80°C
Tags: N-terminal GST-tag
Unit Definition: One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30°C.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Frangioni et al., Cell, 1992, 68: 545.
2. M. Elchebly et al., Science 1999, 283: 1544.
| Artikelnummer | BPS30011 |
|---|---|
| Hersteller | BPS Bioscience |
| Hersteller Artikelnummer | 30011 |
| Green Labware | Nein |
| Verpackungseinheit | 50 µg |
| Mengeneinheit | STK |
| Produktinformation (PDF) | Download |
| MSDS (PDF) |
|
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Gernot Ensinger, M.Sc.
Laurent Haze
Laurent Haze