c-RAF, GST-Tag Recombinant

Products from BPS Bioscience require a minimum order value above 400€

Applications: Useful for studying RAS/RAF binding study as well as inhibitor screening for RAS/RAF interaction.

Assay Conditions: GppNHp (or GDP) loaded His-tag KRAS(G12C) at various concentrations (BPS Bioscience #100641 and BPS Bioscience #100640) was incubated with 2.5 nM GST-tag RBD-RAF1 for 1 hour at room temperature. KRAS(G12C)/RBD-RAF1 binding was analyzed by AlphaScreening using GSH-Acceptor beads and Nickle Donor beads (PerkinElmer).

Description: Human Ras Binding Domain (RBD) of Raf-1 Proto-Oncogene, Serine/Threonine Kinase (RAF1) also known as C-Raf and NS5 with N-terminal GST-tag, GenBank Accession No. NM_002880, a.a. 50-140, expressed in an E. coli expression system. MW=36 kDa.

Format: Aqueous buffer solution

Formulation: 40 mM Tris-HCl pH 8.0, 110 mM NaCl, 2.2 mM KCl, 20% glycerol.

Genbank: NM_002880

Storage Stability: At least 6 months at -70°C.

Supplied As: Aqueous buffer solution.

Tags: N-terminal GST-tag

Uniprot: P04049

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: 1. Rooij, J., et al., Oncogene. 1997;14:623-625.
2. Bhaswati, P., et al., Nature Genetics. 2007;39:1007-1012.

• Artikelnummer: BPS100519-2
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 100519-2
• Green Labware: Nein
• Verpackungseinheit: 1 mg
• Mengeneinheit: STK