Rbx1/Cul4B/Dcaf11/Ddb1 Complex Recombinant

Products from BPS Bioscience require a minimum order value above 400€

Background: Eukaryotic cells have evolved complex mechanisms to regulate cellular pathways and protein quality. Cullin proteins are a family of hybrophobic proteins that serve as scaffold and can complex with RING and adaptor proteins to form CRLs (E3 ligase-Cullin-RING ligase) complexes, which can interact with different substrates and regulate multiple cellular processes. When the CRL Rbx1-Cul4b-Ddb1 binds to Dcaf11 it can target p21 for ubiquitination and regulate cell cycle progression.

Description: Complex of Rbx1 (Ring-Box 1), Cul4B (cullin 4B), Dcaf11 (Ddb1- and Cul4-associated factor 11) and Ddb1 (Damage specific DNA binding protein 1. Recombinant Rbx1, encompassing amino acids 2-108(end), mutated at M5I, contains an N-terminal His-tag (6xHis). Recombinant Cul4B, encompassing amino acids 3-913(end), contains an N-terminal His-tag (6xHis). Recombinant Dcaf11, encompassing amino acids 2-546(end), contains an N-terminal FLAG-tag. Recombinant human Ddb1, encompassing amino acids 2-1140(end) contains an N-terminal FLAG-tag. These recombinant proteins were co-expressed, and affinity purified.

Format: Aqueous buffer solution.

Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, and 20% glycerol

Genbank: Rbx1: NM_014248; Cul4B: NM_003588.4; Dcaf11: NM_025230.5; Ddb1: NM_001923

Host Cell Line: HEK293

Purity: ≥85%

Storage Stability: At least 6 months at -80°C.

Warnings: Avoid freeze/thaw cycles

Biosafety Level: Not applicable (BSL-1)

• Artikelnummer: BPS101495-1
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 101495-1
• Green Labware: Nein
• Verpackungseinheit: 10 µg
• Mengeneinheit: STK
• Wirt: Human