Rbx1/CUL4B/DDB1/DCAF15/DDA1 Complex Recombinant

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Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 

Assay Conditions: Assay was performed according to DCAF15 TR-FRET Assay Kit with DCAF15 titrated up to 50 nM.

Description: Recombinant Recombinant human complex of RBx1 (ring-box 1), CUL4B (cullin 4B), DBB1 (damage specific DNA binding protein 1), DCAF15 (DDB1 and CUL4 associated factor 15), and DDA1 (DET1 and DDB1 associated 1). RBx1, full length, encompassing amino acids 2-108(end). This construct contains an N-terminal His-tag (6xHis). CUL4B, encompassing amino acids 3-913(end). This construct contains an N-terminal His-tag (6xHis). DBB1, full length, encompassing amino acids 2-1140(end). This construct contains an N-terminal Flag-tag. DCAF15, full length, encompassing amino acids 2-600(end). This construct contains an N-terminal Flag-tag. DDA1, full length, encompassing amino acids 2-102(end). This construct contains an N-terminal Flag-tag. These recombinant proteins are co-expressed in a HEK293 expression system and affinity purified.

Format: Aqueous buffer solution 

Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol

Genbank: DDB1: NM_001923; CUL4B: NM_003588.4; DCAF15: NM_138353.4; DDA1: NM_024050.6; RBX1: NM_014248

Host Cell Line: HEK293

Purity: ≥90%

Storage Stability: At least 6 months at -80°C. 

Tags: N-terminal FLAG-Avi-Tag, N-terminal His-Tag

Uniprot: DDB1: Q16531; CUL4B: Q13620; DCAF15: Q66K64; DDA1: Q9BW61; RBX1: P62877

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

• Artikelnummer: BPS101497-1
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 101497-1
• Green Labware: Nein
• Verpackungseinheit: 10 µg
• Mengeneinheit: STK
• Wirt: Human