Description: PDGFs exist as disulfide-linked dimers comprising two polypeptide chains, PDGF-A and PDGF-B, each weighing between 12.0 and 13.5 kDa. Among the three naturally occurring PDGFs-PDGF-AA, PDGF-BB, and PDGF-AB-all are potent mitogens affecting various cell types, including smooth muscle cells, connective tissue cells, bone and cartilage cells, and certain blood cells. These growth factors are stored in platelet α-granules and released upon platelet activation, contributing to processes like hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubule epithelial cell development. PDGFs engage two distinct signaling receptors known as PDGFR-α and PDGFR-β. PDGFR-α serves as a high-affinity receptor for all three PDGF forms, while PDGFR-β selectively interacts with PDGF-BB and PDGF-AB. Recombinant Human PDGF-AA is a 28.5 kDa disulfide-linked homodimer consisting of two A chains, totaling 250 amino acids.
Published Species: Human, Monkey, Mouse, Rat
Expression System: E. coli
Molecular Weight: 28.5 kda
Class: Recombinant
Endotoxin Concentration: <1 EU/µg
Activity: Determined by the dose-dependent stimulation of the proliferation of Balb/c 3T3 cells. The expected ED50 for this effect is 1.0-3.0 ng/ml.
Form: Lyophilized
Contains: no preservative
Storage Conditions: -20°C
Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses
Caution: For Research Use Only. Not for use in diagnostic procedures.
Gene Symbol: PDGFA
Protein ID: P04085
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