Recombinant Human RAD1 protein (His tag)

Abbreviation: RAD1

Target Synonym: Cell cycle checkpoint protein RAD1;RAD1;DNA repair exonuclease rad1 homolog;Rad1-like DNA damage checkpoint protein

Target Species: Human

Expression Host: E.coli

Fusion Tag: N-His & C-His

UNIProt ID: O60671

Background: Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. Isoform 1 possesses 3'->5' double stranded DNA exonuclease activity.

Sequence: Met 1-Ser 282

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Endotoxin: Please contact us for more information.

• Artikelnummer: ELSPDEH100342-100
• Hersteller: Elabscience Biotechnology
• Hersteller Artikelnummer: ELA-PDEH100342-100
• Verpackungseinheit: 100 µg
• Mengeneinheit: STK