Description: TNF-α, a versatile pro-inflammatory cytokine, is produced by various cell types such as adipocytes, activated monocytes, macrophages, B cells, T cells, and fibroblasts. It belongs to the TNF ligand family and interacts with two receptors, TNFR1 and TNFR2. Its functions include cytotoxicity against numerous tumor cells and essential involvement in the immune response against bacterial infections. TNF-α is also implicated in septic shock, autoimmune conditions, rheumatoid arthritis, inflammation, and diabetes. Both human and murine TNF-α exhibit substantial reactivity across species. Structurally, TNF-α exists in two forms: a type II transmembrane protein and a mature soluble protein. The transmembrane protein undergoes proteolytic cleavage to generate a biologically active 17 kDa TNF-α, forming a non-covalently linked homotrimer in solution. Recombinant Human TNF-α is a soluble 157 amino acid protein (17.4 kDa) representing the C-terminal extracellular domain of the full-length transmembrane protein.
Expression System: E. coli
Molecular Weight: 17.4 kDa
Class: Recombinant
Endotoxin Concentration: <1 EU/µg
Activity: The ED50 as determined by the cytolysis of murine L929 cells in the presence of Actinomyocin D is ≤ 0.05 ng/ml, corresponding to a specific activity of ≥ 2 x 10^7 units/mg.
Form: Lyophilized
Contains: no preservative
Storage Conditions: -20°C
Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses
Caution: For Research Use Only. Not for use in diagnostic procedures.
Gene Symbol: TNF
Protein ID: P01375
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