Red Blood Cell Depletion Magnetic Beads

Application Note: Red Blood Cell Depletion Magnetic Beads have been tested in binding assays for separation of Red Blood Cells, from whole blood. Remaining white blood cells can be used for downstream assays such as immunoassays, Western blots, FACS, and immunohistochemistry. For 100 µL of whole, fresh blood cells add 75 µL of Depletion beads, expecting 75 – 95% depletion of RBC after one pass. Optimal amount for applications should be determined by the researcher.

Concentration Value: 20 mg/mL

Flow Cytometry Dilution: User Optimized

Western Blot Dilution: User Optimized

General Disclaimer Note: This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen: Human washed pooled Red Blood Cells (RBC)

Physical State: Suspension of Magnetic Beads

Background: Red Blood Cell Depletion Magnetic Beads recognizes human red blood cells. Red Blood Cell Depletion Magnetic Beads is recommended for the depletion of human Red Blood Cells in samples prior to downstream immunological and non-immunological assays and has been tested in Flow Cytometry. Some additional assays could include Colony Forming Unit (CFU) Assay and Immunohistochemistry among others. These magnetic beads are uniform, non-aggregating, super-paramagnetic beads consisting of a ferric oxide core functionalized with various silane groups. The beads have a large surface area with high capture efficiencies. Bead mean diameter is ~0.5 μm, bead concentration is 20 mg/mL.

Low Endotoxin: No

Other: User Optimized

• Artikelnummer: ROC01-1801-20
• Hersteller: Rockland
• Hersteller Artikelnummer: 01-1801-20
• Verpackungseinheit: 2.0 mL
• Mengeneinheit: STK
• Klonalität: Polyclonal
• Isotyp: IgG
• Wirt: Rabbit
• Konjugat: Conjugated