Application Note: Connective Tissue Growth Factor Recombinant Protein has been tested by SDS-PAGE and biological assay and is suitable as a control for polyclonal or monoclonal anti-Connective Tissue Growth Factor in immunological assays.
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Other Performance Data: Endotoxin Level: Measured by kinetic LAL analysis and is typically ≤ 1 EU/μg protein. Biologic Activity: The activity is determined by the dose-dependent stimulation of HUVEC proliferation and is typically 1-2 μg/mL.
Physical State: Lyophilized
Purity and Specificity: Connective Tissue Growth Factor purity was determined to be greater than 98% as determined by analysis by UV-Spectroscopy at 280nm and by reducing and non-reducing SDS-pAGE.
Background: Connective Tissue Growth Factor (CTGF) is a member of cysteine rich regulatory proteins that are both mitogenic and chemotactic. Each protein has an Insulin-like Growth Factor (IGF)-binding domain, a thrombospondin type 1 domain and cysteine knot region. CTGF has multiple effects on development and differentiation. Recombinant human CTGF is a non-glycosylated protein, containing 98 amino acids, with a molecular weight of 11.2 kDa.
Low Endotoxin: Yes
Other: User Optimized