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RIPK2, GST-tag Recombinant
RIPK2, GST-tag Recombinant
Artikelnummer
BPS40173
Verpackungseinheit
10 µg
Hersteller
BPS Bioscience
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Encompassing Amino Acids: 1-299
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Assay Buffer: 25mM MOPS, pH 7.2, 12.5mM β-glycerophophate, 25mM MgCl2, 5mM EGTA, 2mM EDTA.Incubate RIPK2 with substrate (0.2mg/ml) and [32P]-ATP at 30°C for 15 minutes, then spot reaction on P81 paper, fix in 1% phosphoric acid, and assay with a scintillation counter.
Background: RIPK2 (RIP2; RICK) is a death domain-containing protein kinase encoding a predicted 540-amino acid protein which contains an N-terminal serine/threonine kinase catalytic domain and a C-terminal caspase activation and recruitment domain. RIPK2 is thought to regulate apoptosis induced by the FAS receptor pathway (1). RIPK2 has been shown to specifically interact with the CARD of ICE/caspase-1 and this interaction correlates with the processing of pro-caspase-1 and the formation of the active caspase-1 p20 (2).
Description: Recombinant human RIPK2(GenBank Accession No. NM_003821), (a.a. 1-299) with N-terminal Gst-tag,MW=59 kDa, expressed in an baculovirusinfected Sf9 cell expression system.
Format: Aqueous buffer solution
Formulation: 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol.
Genbank: NM_003821
Host Cell Line: Sf9 cells
Purity: ≥75%
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: O43353
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Inohara, N. et al: RICK, a novel protein kinase containing a caspase recruitment domain, interacts with CLARP and regulates CD95-mediated apoptosis. J. Biol. Chem. 273: 12296-12300, 1998. Note: Erratum: J. Biol. Chem 273: 18675 only, 1998.
2. Thome, M. et al: Identification of CARDIAK, a RIP-like kinase that associates with caspase-1. Curr. Biol. 8: 885-888, 1998.
Encompassing Amino Acids: 1-299
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Assay Buffer: 25mM MOPS, pH 7.2, 12.5mM β-glycerophophate, 25mM MgCl2, 5mM EGTA, 2mM EDTA.Incubate RIPK2 with substrate (0.2mg/ml) and [32P]-ATP at 30°C for 15 minutes, then spot reaction on P81 paper, fix in 1% phosphoric acid, and assay with a scintillation counter.
Background: RIPK2 (RIP2; RICK) is a death domain-containing protein kinase encoding a predicted 540-amino acid protein which contains an N-terminal serine/threonine kinase catalytic domain and a C-terminal caspase activation and recruitment domain. RIPK2 is thought to regulate apoptosis induced by the FAS receptor pathway (1). RIPK2 has been shown to specifically interact with the CARD of ICE/caspase-1 and this interaction correlates with the processing of pro-caspase-1 and the formation of the active caspase-1 p20 (2).
Description: Recombinant human RIPK2(GenBank Accession No. NM_003821), (a.a. 1-299) with N-terminal Gst-tag,MW=59 kDa, expressed in an baculovirusinfected Sf9 cell expression system.
Format: Aqueous buffer solution
Formulation: 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol.
Genbank: NM_003821
Host Cell Line: Sf9 cells
Purity: ≥75%
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: O43353
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Inohara, N. et al: RICK, a novel protein kinase containing a caspase recruitment domain, interacts with CLARP and regulates CD95-mediated apoptosis. J. Biol. Chem. 273: 12296-12300, 1998. Note: Erratum: J. Biol. Chem 273: 18675 only, 1998.
2. Thome, M. et al: Identification of CARDIAK, a RIP-like kinase that associates with caspase-1. Curr. Biol. 8: 885-888, 1998.
| Artikelnummer | BPS40173 |
|---|---|
| Hersteller | BPS Bioscience |
| Hersteller Artikelnummer | 40173 |
| Green Labware | Nein |
| Verpackungseinheit | 10 µg |
| Mengeneinheit | STK |
| Wirt | Insect |
| Produktinformation (PDF) | Download |
| MSDS (PDF) |
|
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