Target Synonym: NML; KIAA0409
Storage: Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Background: Essential component of the eNoSC (energy-dependent nucleolar silencing complex, a complex that mediates silencing of rDNA in response to intracellular energy status and acts by recruiting histone-modifying enzymes. The eNoSC complex is able to sense the energy status of cell: upon glucose starvation, elevation of NAD(+/NADP(+ ratio activates SIRT1, leading to histone H3 deacetylation followed by dimethylation of H3 at 'Lys-9' (H3K9me2 by SUV39H1 and the formation of silent chromatin in the rDNA locus. In the complex, RRP8 binds to H3K9me2 and probably acts as a methyltransferase. Its substrates are however unknown.
Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Immunogen: A synthetic peptide of human RRP8
Dilution: WB 1:500-1:2000
Calculated MW: 50kDa
Observed MW: 58KDa
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