SARS-CoV-2 Spike Trimer (S1+S2):ACE2 Inhibitor Screening Colorimetric Assay Kit

Products from BPS Bioscience require a minimum order value above 400€

Applications: This kit is useful for screening for inhibitor of ACE2 binding to trimeric SARS-CoV-2 Spike

Description: The pandemic coronavirus disease 2019 (COVID-19) is caused by Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). The Spike glycoprotein is expressed on the surface of the virus as a trimer. Each Spike protein consists of two subunits, S1 and S2, and the S1 subunit has a receptor binding domain (RBD) which recognizes and attaches to the Angiotensin-Converting Enzyme 2 (ACE2) receptor found on the surface of type I and II pneumocytes, endothelial cells, and ciliated bronchial epithelial cells. Drugs targeting the interaction between the Spike protein of SARS-CoV-2 and ACE2 may offer some protection against the viral infection. The SARS-CoV-2 Spike Trimer (S1+S2) :ACE2 Inhibitor Screening Assay Kit includes the Spike protein in its native trimeric conformation to provide a more physiologically relevant screen for inhibitors of the Spike S1:ACE2 interaction.The SARS-CoV-2 Spike Trimer (S1+S2) :ACE2 Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of this interaction. The key to this kit is that the SARS-CoV-2 Spike Trimer (S1+S2) protein provides a more biologically relevant model than monomeric Spike RBD protein for the investigation of SARS-CoV-2/host cell interaction. Only a few simple steps on a microtiter plate are required for the assay. First, SARS-CoV-2 Spike Trimer (S1+S2) is coated on a 96-well plate. Next, Biotin-ACE2 is incubated with SARS-CoV-2 Spike Trimer (S1+S2) on the plate. Finally, the plate is treated with Streptavidin-HRP followed by addition of a colorimetric HRP substrate to produce color, which then can be quenched and measured using a UV/Vis microplate reader.

Storage Stability: Up to 6 months from date of receipt, when stored as recommended.

Supplied As: This kit comes in a convenient 96-well format, with Biotinylated-ACE2, purified SARS-CoV-2 Spike Trimer (S1+S2) protein, Streptavidin-HRP, and assay buffers for 100 binding reactions.

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: Hoffmann, M. et al. 2020. Cell, 181:1-10
Yan, R. et al. 2020. Science 367 (6485): 1444-1448.

• Artikelnummer: BPS79999
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 79999
• Green Labware: Nein
• Verpackungseinheit: 96 rxns.
• Mengeneinheit: PAK