Sars-Cov Nonstructural Protein 13 Antibody

Application Note: This antibody is suitable for use in western blotting, immunofluorescence microscopy and immunoelectron microscopy. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 67 kDa in size corresponding to SARS-CoV nsp13 by western blotting in the appropriate cell lysate or extract. For immunofluorescence microscopy, Vero-E6 cells, grown on glass slides, were infected with SARS-CoV-Fr1 strain for 1 h at 37°C.  Infection occurred in PBS/DEAE/2% FCS followed by exchange to EMEM/25mMHEPES/2% FCS. Cells were fixed with PBS/3% PFA.  After washing fixed cells, antibody incubation was performed in PBS/5% FCS for 30 min.

Concentration Value: 85 mg/mL

IF Microscopy Dilution: 1:800

Immunoprecipitation Dilution: 1:60

Western Blot Dilution: 1:1,000

General Disclaimer Note: This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen: This antibody was prepared from whole rabbit serum produced by repeated immunizations with a BSA-coupled synthetic peptide corresponding to the C-terminus (amino acid residues 584-601) of the SARS Coronavirus nonstructural protein 13.

Physical State: Liquid (sterile filtered)

Purity and Specificity: This antibody is directed against SARS-Coronavirus nsp13 protein. The product is neat antiserum. Cross reactivity with homologues from other sources has not been determined.

Background: The coronavirus nonstructural protein 13 (nsp13) is one of the SARS-Coronavirus replicase cleaving products encoded by ORF1b. Nsp13 is thought to be part of the viral replication complex, which is associated with intracellular membranes. Nsp13 contains a C-terminal NTPase/Helicase domain and an N-terminal putative zinc-binding motif. Anti-SARS-CoV Nonstructural Protein 13 (nsp13) Antibody is useful for researchers interested in viral research.

Low Endotoxin: No

Other: IMMUNOELECTRON MICROSCOPY 1:300

• Artikelnummer: ROC100-401-A54
• Hersteller: Rockland
• Hersteller Artikelnummer: 100-401-A54
• Verpackungseinheit: 100 µL
• Mengeneinheit: STK
• Reaktivität: Virus
• Klonalität: Polyclonal
• Isotyp: Antiserum
• Human Gene ID: 1489680
• Wirt: Rabbit
• Konjugat: Unconjugated