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Applications: Monitor TGFβ signaling pathway activity. Screen for activators or inhibitors of the TGFβ/ SMAD signaling pathway. Study effects of RNAi or gene overexpression on the activity of the TGFβ pathway.
Concentration: Both the Reporter and Negative Control Reporter are provided at 60 ng DNA/µl (500 µl each)
Description: The SBE Reporter kit is designed for monitoring the activity of TGFβ/SMAD signaling pathway in the cultured cells. The kit contains transfection-ready SBE luciferase reporter vector, which is a TGFβ pathway-responsive reporter. This reporter contains a firefly luciferase gene under the control of multimerized SBE responsive element located upstream of a minimal promoter. The SBE reporter is premixed with constitutively-expressing Renilla luciferase vector that serves as internal control for transfection efficiency. The kit also includes a non-inducible firefly luciferase vector premixed with constitutively-expressing Renilla luciferase vector as negative control. The noninducible luciferase vector contains a firefly luciferase gene under the control of a minimal promoter, without any additional response elements. The negative control is critical to determining pathway-specific effects and background luciferase activity.
Format: assay kit
Storage Stability: Stable at least 12 months from date of receipt, when stored as directed (-20°C)