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SGK3, GST-tag Recombinant
SGK3, GST-tag Recombinant
Artikelnummer
BPS40273
Verpackungseinheit
10 µg
Hersteller
BPS Bioscience
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Encompassing Amino Acids: full length
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: 5 mM MOPS, 2.5 mM β-glycerophosphate, 5 mM MgCl2, 1 mM EGTA, 0.4 mM EDTA, 0.05 mM DTT, 2 mM ATP. Incubate SGK3 with 0.2 mg/ml Akt (PKB) peptide substrate (CKRPRAASFAE) and 50 ?M [33P]-ATP at 30°C for 15 minutes, then spot reaction on phosphocellulose paper, fix in 1% phosphoric acid, and assay with a scintillation counter.
Background: SGK3 is a member of the SGK family and encodes a phosphoprotein with a PX (phox homology) domain. PKD1 can phosphorylate and activate SGK3 in vitro (1). A 10-fold increase in PKD1 increases the phosphorylation status of SGK3. When expressed in human embryonic kidney cells, IGF1 and peroxide significantly activate SGK3, and the activation could be reduced by preincubation with inhibitors of PI3 kinase. A yeast 2-hybrid screen found direct interaction between human SGK3 and GSK3β. SGK3 phosphorylates several target proteins and has a role in neutral amino acid transport and activation of potassium and chloride channels (2).
Description: Recombinant full-length human SGK3, also known as CISK and SGKL, (Genbank Accession No. NM_013257) with an N-terminal GST-tag, expressed in Sf9 insect cells using a baculovirus expression system.
Format: Aqueous buffer solution
Formulation: 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol.
Genbank: NM_013257
Host Cell Line: Sf9 cells
Purity: ≥75%
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: Q96BR1
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Kobayashi, T. et al: Characterization of the structure and regulation of two novel isoforms of serum- and glucocorticoid-induced protein kinase. Biochem. J. 344: 189-197, 1999.
2. Gamper, N. et al: K+ channel activation by all three isoforms of serum- and glucocorticoid-dependent protein kinase SGK. Europ. J. Physiol. 445: 60-66, 2002.
Encompassing Amino Acids: full length
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: 5 mM MOPS, 2.5 mM β-glycerophosphate, 5 mM MgCl2, 1 mM EGTA, 0.4 mM EDTA, 0.05 mM DTT, 2 mM ATP. Incubate SGK3 with 0.2 mg/ml Akt (PKB) peptide substrate (CKRPRAASFAE) and 50 ?M [33P]-ATP at 30°C for 15 minutes, then spot reaction on phosphocellulose paper, fix in 1% phosphoric acid, and assay with a scintillation counter.
Background: SGK3 is a member of the SGK family and encodes a phosphoprotein with a PX (phox homology) domain. PKD1 can phosphorylate and activate SGK3 in vitro (1). A 10-fold increase in PKD1 increases the phosphorylation status of SGK3. When expressed in human embryonic kidney cells, IGF1 and peroxide significantly activate SGK3, and the activation could be reduced by preincubation with inhibitors of PI3 kinase. A yeast 2-hybrid screen found direct interaction between human SGK3 and GSK3β. SGK3 phosphorylates several target proteins and has a role in neutral amino acid transport and activation of potassium and chloride channels (2).
Description: Recombinant full-length human SGK3, also known as CISK and SGKL, (Genbank Accession No. NM_013257) with an N-terminal GST-tag, expressed in Sf9 insect cells using a baculovirus expression system.
Format: Aqueous buffer solution
Formulation: 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol.
Genbank: NM_013257
Host Cell Line: Sf9 cells
Purity: ≥75%
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: Q96BR1
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Kobayashi, T. et al: Characterization of the structure and regulation of two novel isoforms of serum- and glucocorticoid-induced protein kinase. Biochem. J. 344: 189-197, 1999.
2. Gamper, N. et al: K+ channel activation by all three isoforms of serum- and glucocorticoid-dependent protein kinase SGK. Europ. J. Physiol. 445: 60-66, 2002.
| Artikelnummer | BPS40273 |
|---|---|
| Hersteller | BPS Bioscience |
| Hersteller Artikelnummer | 40273 |
| Green Labware | Nein |
| Verpackungseinheit | 10 µg |
| Mengeneinheit | STK |
| Wirt | Insect |
| Produktinformation (PDF) | Download |
| MSDS (PDF) |
|
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