Sheep anti Guinea Pig IgG (Fc specific), conjugated with Horseradish peroxidase

Sheep anti Guinea Pig IgG (Fc specific), conjugated with Horseradish peroxidase, Polyclonal, Clone: Polyclonal

Clone: Polyclonal

Background: The reactivity of the antiserum is directed to the Fc subunit of the IgG molecule which expresses strict isotypic (class) specificity. It does not react with any non-Ig protein in guinea pig serum, as tested by immunoelectrophoresis and double radial immunodiffusion In enzyme-immunocytochemical and immunohistochemical staining for the detection of IgG at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgG antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of guinea pig origin known to be of the IgG isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgG in guinea pig serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.

Source: Purified normal IgG isolated from pooled guinea pig serum. Freund’s complete adjuvant is used in the first step of the immunization procedure

Specificity: Horseradish peroxidase-conjugated IgG fraction of polyclonal Sheep antiSerum to Guinea Pig IgG, Fc specific.

Formulation: Horseradish peroxidase-coupled purified hyperimmune sheep IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity.

Caution: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.

• Artikelnummer: ShAGp/IgGFcPO
• Hersteller: Nordic-MUbio
• Hersteller Artikelnummer: ShAGp/IgG(Fc)/PO
• Green Labware: Nein
• Verpackungseinheit: 1 ml
• Mengeneinheit: STK
• Reaktivität: Guinea Pig
• Klonalität: Polyclonal
• Methode: Immunohistochemistry (paraffin), Western Blotting, ELISA, Immunocytochemistry, Dot Blot
• Wirt: Sheep