English
Sheep anti Rat alpha foetoprotein
Sheep anti Rat alpha foetoprotein, Polyclonal, Clone: Polyclonal
Artikelnummer
ShARa/AFP/7S
Verpackungseinheit
10 mg
Hersteller
Nordic-MUbio
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Clone: Polyclonal
Background: The reactivity of the antiserum is restricted to alpha-foetoprotein. In immunoelectrophoresis and double radial immunodiffusion, using various antiserum concentrations against amniotic fluid, rat hepatoma sera and purified alpha-foetoprotein an single characteristic precipitin line is obtained. No reaction is obtained with rat sera of different strains. As unlabelled primary or secondary antibody reagent for the indirect detection of alpha foetoprotein in tissues and body fluids in immunofluorescence and immunoenzyme methods; for the production of immunoconjugates with a selected marker; to prepare insoluble immunoaffinity adsorbents by coupling to an artificial carrier; as catching or detection reagent in non-isotopic methodology and solid phase immunochemistry (e.g. ELISA, Western blotting). When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration.
Source: Purified rat alpha-foetoprotein is isolated from pooled amniotic fluid and hepatoma serum using a proprietary multiple step method. Several foetoproteins have been demonstrated in rat foetal serum and amniotic fluid. One of the best studied is the alpha-foetoprotein. It can be easily identified immunochemically because of its distinct antigenicity. In electrophoresis its relative mobility is between the albumin and the alpha-1 globulin fractions. Its molecular weight is 65,000, the carbohydrate content is relatively low. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Specificity: Purified IgG fraction of polyclonal Sheep antiSerum to Rat alpha foetoprotein.
Formulation: Purified hyperimmune sheep IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the activity of the antiserum.
UniProt: P02773
Caution: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
Background: The reactivity of the antiserum is restricted to alpha-foetoprotein. In immunoelectrophoresis and double radial immunodiffusion, using various antiserum concentrations against amniotic fluid, rat hepatoma sera and purified alpha-foetoprotein an single characteristic precipitin line is obtained. No reaction is obtained with rat sera of different strains. As unlabelled primary or secondary antibody reagent for the indirect detection of alpha foetoprotein in tissues and body fluids in immunofluorescence and immunoenzyme methods; for the production of immunoconjugates with a selected marker; to prepare insoluble immunoaffinity adsorbents by coupling to an artificial carrier; as catching or detection reagent in non-isotopic methodology and solid phase immunochemistry (e.g. ELISA, Western blotting). When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration.
Source: Purified rat alpha-foetoprotein is isolated from pooled amniotic fluid and hepatoma serum using a proprietary multiple step method. Several foetoproteins have been demonstrated in rat foetal serum and amniotic fluid. One of the best studied is the alpha-foetoprotein. It can be easily identified immunochemically because of its distinct antigenicity. In electrophoresis its relative mobility is between the albumin and the alpha-1 globulin fractions. Its molecular weight is 65,000, the carbohydrate content is relatively low. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Specificity: Purified IgG fraction of polyclonal Sheep antiSerum to Rat alpha foetoprotein.
Formulation: Purified hyperimmune sheep IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the activity of the antiserum.
UniProt: P02773
Caution: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
| Artikelnummer | ShARa/AFP/7S |
|---|---|
| Hersteller | Nordic-MUbio |
| Hersteller Artikelnummer | ShARa/AFP/7S |
| Verpackungseinheit | 10 mg |
| Mengeneinheit | STK |
| Reaktivität | Rat (Rattus) |
| Klonalität | Polyclonal |
| Methode | Western Blotting, ELISA, Dot Blot, Immunofluorescence (indirect) |
| Wirt | Sheep |
| Konjugat | Unconjugated |
| Produktinformation (PDF) | Download |
| MSDS (PDF) |
|
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Gernot Ensinger, M.Sc.
Laurent Haze
