SHP-2 (PTPN11), GST-tag Recombinant

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Encompassing Amino Acids: 224-529

Applications: Useful for the study of enzyme kinetics and regulation, to dephosphorylate target substrates and for screening inhibitors.

Assay Conditions: 50 mM HEPES, pH 7.4, 2 mM EDTA, 3 mM DTT, 100 mM NaCl, 50 mM pNPP.

Background: Mammalian PTPases can be subdivided into 1 of 2 broad categories: transmembrane receptor PTPases and intracellular PTPases. PTPN11 is one of the 2 closely related mammalian intracellular PTPases whose sequences encode 2 tandem SRC homology 2 (SH2) domains that are located at the amino-terminal side of a single PTPase catalytic domain. This PTP is widely expressed in most tissues and plays a regulatory role in various cell signaling events that are important for a diversity of cell functions, such as mitogenic activation, metabolic control, transcription regulation, and cell migration.

Description: Protein tyrosine phosphatase SHP-2, also known as PTPN11 (GenBank accession number NM_002834), a.a. 224-529 (catalytic domain) with N-terminal GST-tag, MW=62 kDa, expressed in an E. coli expression system.

Format: Aqueous buffer solution

Formulation: 25 mM Tris-HCl, pH 8.0, 75 mM NaCl, 0.05% Tween-20, 50% glycerol, 2 mM EDTA, 1 mM DTT, 10 mM glutathione.

Genbank: NM_002834

Storage Stability: >12 months at -80°C

Tags: N-terminal GST-tag

Uniprot: Q06124

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: 1. B.G. Neel et al. Trends Biochem. Sci. (2003) 28:284.
2. J.V. Ravetch and L.L. Lanier Science (2000) 290:84.
3. S. Yamazaki et al. J. Infect. Dis. (2003) 187:334.

• Artikelnummer: BPS30022
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 30022
• Green Labware: Nein
• Verpackungseinheit: 50 µg
• Mengeneinheit: STK