SIRP-α / HEK293 Recombinant Cell Line

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Applications: This cell line is useful for SIRPα:CD47 protein binding analyses and screening for antibodies or inhibitors of CD47.

Background: SIRPα (CD172A, PTPNS1) is a member of the signal-regulatory-protein (SIRP) family, which are transmembrane immunoglobulin receptors that negatively regulate receptor tyrosine kinase-coupled signaling processes. SIRPα is expressed predominantly on macrophages and dendritic cells. Interaction with its ligand CD47 mediates signals that blocks phagocytosis (also known as the "don't eat me" signal).

Description: Recombinant HEK293 cell constitutively expressing full length human SIRPα (#NM_080792). Surface expression is confirmed by flow cytometry.

Format: Each vial contains ~2 x 10^6 cells in 1 ml of 10% DMSO in FBS.

Host Cell Line: HEK293

Mycoplasma Testing: This cell line has been screened using the MycoAlert (Cat. #LT07-118) to confirm the absence of Mycoplasma contamination. MycoAlert Assay Control Set (Cat # LT07-518) was used as a positive control.

Storage Stability: Store in liquid nitrogen immediately upon receipt.

Biosafety Level: BSL-2

References: 1.Lee WY et.al. (2010) The Role of cis Dimerization of Signal Regulatory Protein a (SIRPa) in Binding to CD47. J. Biol. Chem. 285: 37953-37963

• Artikelnummer: BPS60689
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 60689
• Verpackungseinheit: 2 vials
• Mengeneinheit: PAK
• Wirt: Human