Sirtuin 1 (193-741), GST-tag Recombinant

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Encompassing Amino Acids: 193- 741

Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 

Assay Conditions: 25 mM Tris/HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl₂, and 0.1 mg/ml BSA, 20 µM BPS HDAC substrate 1 (Catalog #50032), 500 µM NAD+ and Sirtuin 1 (12.5 - 800 ng). Incubation condition: 30 min at 37°C followed by treatment with 50 µl of SIRT developer (Catalog # 50089) for 15 min at RT. Fluorescence intensity is measured at ex360/em460.

Description: Human Sirtuin 1 , GenBank Accession No. NM_012238, a.a. 193-741 with N-terminal GST-tag, MW= 87.2 kDa, expressed in an E. coli expression system.

Format: Aqueous buffer solution

Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 16 mM glutathione, 20% glycerol

Genbank: NM_012238

Storage Stability: At least 6 months at -80°C.

Tags: N-terminal GST-tag

Uniprot: Q96EB6

Warnings: Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended. If necessary, use carrier protein (BSA 0.1 - 0.5%).

Biosafety Level: Not applicable (BSL-1)

References: 1. Kolthur-Seetharam, U. et al (2006) Cell Cycle 5, 873-877.
2. Solomon, J.M. et al (2006) Mol. Cell. Biol. 26, 28-38.
Application References
1. Arcand, M., et al. "Development of High-Throughput Assays to Study Methylases, Demethylases and Deacetylases." Posterpresented as part of the BioTechniques Fall 2011 Epigenetics Meeting.

• Artikelnummer: BPS50012
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 50012
• Green Labware: Nein
• Verpackungseinheit: 100 µg
• Mengeneinheit: STK