Sirtuin 2, His-Tag Recombinant

Products from BPS Bioscience require a minimum order value above 400€

Encompassing Amino Acids: 13-319

Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 

Assay Conditions: 25 mM Tris/HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl₂, and 0.1 mg/ml BSA, 20 µM BPS HDAC substrate 1 (Catalog #50032), 500 µM NAD+ and Sirtuin 2 (125 - 8000 ng). Incubation condition: 30 min at 37°C followed bytreatment with 50 µl of SIRT developer (Catalog # 50089) for 15 min at RT. Fluorescence intensity is measured at ex360/em460.

Description: Human Sirtuin 2, GenBank Accession No. NM_012237, a.a. 50-356 with C-terminal His-tag, MW= 35.5 kDa, expressed in an E. coli expression system.

Format: Aqueous buffer solution

Formulation: 40 mM Tris pH 8.0, 110 mM NaCl, 2.1 mM KCl, and 20% glycerol.

Genbank: NM_012237

Storage Stability: At least 6 months at -80°C.

Tags: C-terminal His-tag

Uniprot: Q8IXJ6

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: 1. Grubisha, O., 2006, J.Biol.Chem. 281, 20, 14057-65.
2. Vaquero, A., 2006, Genes Dev. 20, 10, 1256- 61.Product Specific Reference(s):
1. Nguyen, P., 2014, Mol Cancer Res. 12, 9, 1244-53.

• Artikelnummer: BPS50013
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 50013
• Green Labware: Nein
• Verpackungseinheit: 100 µg
• Mengeneinheit: STK