English
SLK, GST-tag Recombinant
SLK, GST-tag Recombinant
Artikelnummer
BPS40275
Verpackungseinheit
10 µg
Hersteller
BPS Bioscience
Verfügbarkeit:
wird geladen...
Preis wird geladen...
Products from BPS Bioscience require a minimum order value above 400€
Encompassing Amino Acids: full length
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Background: SLK or LOSK is a Ste20-like protein kinase that associates with microtubule and centrosome. Inhibition of SLK activity by dominant-negative mutant or RNAi leads to unfocused microtubule arrangement indicating that SLK is needed for microtubule organization and for the proper localization of Golgi complex (1). Expression of v-Src can down-regulate SLK activity through the involvement of CK2 which can directly phosphorylate and inhibit SLK activity whereas inhibition of CK2 in v-Src-transformed cells results in normal SLK activity (2). CK2 and SLK can be co-localized in fibroblasts spreading on fibronectin-coated substrates.
Description: Recombinant human SLK, full length, with N-terminal GST-tag, expressed in Sf9 insect cells via a baculovirus expression system.
Format: Aqueous buffer solution
Formulation: 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol
Genbank: AB002804
Host Cell Line: Sf9 cells
Purity: ≥80%
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: Q9H2G2
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Burakov A V, et al: LOSK (SLK) protein kinase activity is necessary for microtubule organization in the interphase cell centrosome. Dokl Biol Sci. 2005;403:317-9.
2. Chaar Z, et al: v-Src-dependent down-regulation of the Ste20-like kinase SLK by casein kinase II. J Biol Chem. 2006 Sep 22;281(38):28193-9.
Encompassing Amino Acids: full length
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Background: SLK or LOSK is a Ste20-like protein kinase that associates with microtubule and centrosome. Inhibition of SLK activity by dominant-negative mutant or RNAi leads to unfocused microtubule arrangement indicating that SLK is needed for microtubule organization and for the proper localization of Golgi complex (1). Expression of v-Src can down-regulate SLK activity through the involvement of CK2 which can directly phosphorylate and inhibit SLK activity whereas inhibition of CK2 in v-Src-transformed cells results in normal SLK activity (2). CK2 and SLK can be co-localized in fibroblasts spreading on fibronectin-coated substrates.
Description: Recombinant human SLK, full length, with N-terminal GST-tag, expressed in Sf9 insect cells via a baculovirus expression system.
Format: Aqueous buffer solution
Formulation: 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol
Genbank: AB002804
Host Cell Line: Sf9 cells
Purity: ≥80%
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: Q9H2G2
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Burakov A V, et al: LOSK (SLK) protein kinase activity is necessary for microtubule organization in the interphase cell centrosome. Dokl Biol Sci. 2005;403:317-9.
2. Chaar Z, et al: v-Src-dependent down-regulation of the Ste20-like kinase SLK by casein kinase II. J Biol Chem. 2006 Sep 22;281(38):28193-9.
| Artikelnummer | BPS40275 |
|---|---|
| Hersteller | BPS Bioscience |
| Hersteller Artikelnummer | 40275 |
| Green Labware | Nein |
| Verpackungseinheit | 10 µg |
| Mengeneinheit | STK |
| Wirt | Insect |
| Produktinformation (PDF) |
|
| MSDS (PDF) |
|
Immer für Sie da
Gernot Ensinger, M.Sc.
Laurent Haze
