Spike S1 (13-665), Fc fusion, Avi-tag, Biotin-Labeled (SARS-CoV-2) Recombinant

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Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 

Assay Conditions: Nickel plate wells were coated with 1µg/ml ACE2, His-Tag (BPS Bioscience, #11003, 50 ng/well). Then, 2019-nCoV Spike S1, Fc-fusion (IgG1), Biotin-labeled was incubated with ACE2, His-tag for 1 hour at room temperature, 50µl/well. Bound protein was incubated with Streptavidin-HRP (BPS Bioscience, #79742) and luminescence was measured in a BioTek Synergy 2 microplate reader.

Description: Human Severe acute respiratory Coronavirus 2 Spike Glycoprotein S1 (SARS-CoV-2 Spike S1), GenBank Accession No. QHD43416.1, a.a. 13-665, fused at the C-terminus of the Fc portion of human IgG1, with a C-terminal Avi-tag, expressed in a HEK293 expression system and enzymatically biotinylated using Avi-tag™ technology. Biotinylation is confirmed to be ≥90%. MW=102 kDa. This protein runs at a higher MW by SDS-PAGE due to glycosylation.

Formulation: 8 mM Phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol.

Genbank: QHD43416.1

Storage Stability: At least 6 months at -80°C.

Supplied As: Aqueous buffer solution.

Tags: C-terminal Fc-Avi-tag

Uniprot: P0DTC2 SPIKE_WCPV

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: 1. Hoffman, M. et al., Cell. 2020 Apr; 181:1-10.
2. Walls, A. C. et al., Cell. 2002 Mar; 180:1-12

• Artikelnummer: BPS100679-1
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 100679-1
• Green Labware: Nein
• Verpackungseinheit: 20 µg
• Mengeneinheit: STK