Spike S1 (16-685), Fc Fusion, Avi-tag (SARS-CoV-2) Recombinant

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Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 

Assay Conditions: SARS-CoV-2 S1 (BPS Bioscience #100719) and ACE2 binding assay was done using BPS ACE2:SARS-CoV-2 Spike Inhibitor Screening Assay Kit (BPS Bioscience #79936). SARS-CoV-2 S1 (BPS Bioscience #100719) protein and anti-human Fc-HRP were used instead of SARS-CoV-2 Spike protein (RBD) and anti-mouse Fc-HRP respectively.

Description: Severe acute respiratory Coronavirus 2 Spike Glycoprotein S1 (SARS-CoV-2 Spike S1), GenBank Accession No. QHD43416.1, a.a. 16-685, fused at the C-terminus of the Fc portion of human IgG1, with a C-terminal Avi-tag™, expressed in a HEK293 expression system, MW=104 kDa. This protein runs at a higher MW by SDS-PAGE due to glycosylation.

Formulation: 8 mM Phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol.

Genbank: QHD43416.1

Storage Stability: At least 6 months at -80°C.

Supplied As: Aqueous buffer solution.

Tags: C-terminal Fc-Avi-tag

Uniprot: P0DTC2 SPIKE_WCPV

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: 1. Hoffman, M. et al., Cell. 2020 Apr; 181:1-10
2. Walls, A. C. et al., Cell. 2002 Mar; 180:1-12

• Artikelnummer: BPS100719-1
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 100719-1
• Green Labware: Nein
• Verpackungseinheit: 100 µg
• Mengeneinheit: STK