Spike S1 (B.1.1.529 BA.1, Omicron Variant), Avi-His-Tag, Biotin-Labeled (SARS-CoV-2) Recombinant

Products from BPS Bioscience require a minimum order value above 400€

Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 

Assay Conditions: Assay run according to the Spike S1 (B.1.1.529 BA.1, Omicron Variant) (SARS-CoV-2): ACE2 TR-FRET Assay Kit (BPS Bioscience #78358). 5 ng of ACE2-Eu, 400-fold diluted Dye-labeled acceptor, and various amounts of Spike S1[B] incubated for 1 hour at room temperature. TR-FRET measured at ex340 ± 20nm, em620 ± 10 nm; and ex340 ± 20nm, em665 ± 10 nm.

Description: Recombinant SARS-CoV-2 Spike protein S1 subunit, encompassing amino acids 16-685. This protein corresponds to SARS-CoV-2 Variant B.1.1.529 BA.1, also known as variant Omicron BA.1 originally identified in South Africa, and contains genetic alterations indicated below. It was constructed with a C-terminal Avi-Tag™ followed by a His-tag (6xHis).  The protein was enzymatically biotinylated using the Avi-Tag™ and affinity purified.

Format: Aqueous buffer solution

Formulation: 8 mM phosphate pH 7.4, 110 mM NaCl, 2.2 mM KCl, 20% glycerol

Genbank: MN908947

Purity: ≥90%

Storage Stability: At least 6 months at -80°C.

Tags: C-terminal Avi-His-tag

Uniprot: P0DTC2

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

• Artikelnummer: BPS101334-1
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 101334-1
• Green Labware: Nein
• Verpackungseinheit: 25 µg
• Mengeneinheit: STK