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Applications: ELISA and neutralization assays.
Background: This antibody was derived from COVID-19 patients who have cleared the virus. Patient serum IgG was sequenced and expressed as full-length IgG1 with human immunoglobulin heavy and light chains in mammalian 293 cells.
Description: Recombinant human monoclonal (clone 414-1) antibody recognizing the SARS-CoV-2 Spike S1 RBD glycoprotein. This antibody cross-reacts with the Spike protein from the SARS-CoV virus.
Format: Aqueous buffer solution
Formulation: 140 mM HEPES, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 0.035% sodium azide, and 30% glycerol.
Genbank: QHD43416.1
Purification: Protein A Chromatography
Storage Stability: Stable for at least 12 months at -20°C.
Supplied As: Aqueous buffer solution.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Zhou P., et al., Nature. 2020; 579: 270-289.
2. Xiao X., et al., Cell Mol Life Sci. 2004; 61(19-20): 2428-2430.