Spike S1 Neutralizing Antibody (VHH), Fc-fusion (IgG1), Avi-Tag (SARS-CoV-1)

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Encompassing Amino Acids: 1-127(full length)

Applications: ELISA and neutralization assays.

Assay Conditions: Inhibition of ACE2:SARS-CoV-1 Spike binding by a small anti-SARS-CoV-1 Spike antibody, VHH (BPS Bioscience, #100784). A small anti-SARS-CoV-1 Spike antibody was evaluated using the SARS-CoV-1 Spike Trimer (S1+S2):ACE2 Inhibitor Screening Colorimetric Assay Kit (BPS Bioscience, #79999). The antibody was serially diluted in 1x Immuno Buffer (BPS Bioscience, #79311) and tested following the assay kit protocol.

Description: SARS-CoV monoclonal Spike S1 VHH neutralizing antibody, a.a. 1-127(full length), with C-terminal Avi-Tag™ fused to the Fc portion of Human IgG1, expressed in a HEK293 cell expression system. MW = 43 kDa. VHHs are single-domain antibodies from camelids.

Format: Aqueous buffer solution

Formulation: 8 mM Phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol.

Purification: Protein A Chromatography

Storage Stability: Stable for at least 6 months at -80°C.

Supplied As: Aqueous buffer solution.

Tags: C-terminal Fc-Avi-Tag

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: 1. Ho, M. et al., Antibody Therapeutics. 2020; 3(2): 109-114
2. Wrapp, D. et al., 2020. Cell 181: 1004-1015

• Artikelnummer: BPS100784
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 100784
• Green Labware: Nein
• Verpackungseinheit: 100 µg
• Mengeneinheit: STK
• Klonalität: Monoclonal
• Methode: ELISA
• Isotyp: IgG1