Spike S1 Neutralizing Antibody (VHH), Fc-fusion (IgG1), Avi-Tag (SARS-CoV-1), Biotin-labeled

Products from BPS Bioscience require a minimum order value above 400€

Applications: ELISA and neutralization assays.

Assay Conditions: Spike Trimer (S1+S2), His-tag (SARS-CoV-1) (BPS Bioscience, #100789) and Spike S1 Neutralizing Antibody (VHH), Fc-fusion (IgG1), Avi-Tag (SARS-CoV-1),Biotin-labeled binding was measured by ELISA using Strepavidin-HRP and Colorimetric HRP substrate.

Description: SARS-CoV-1 monoclonal Spike S1 VHH neutralizing antibody, a.a. 1-127(full length), with C-terminal Avi-Tag fused to the Fc portion of Human IgG1, expressed in a HEK293 cell expression system. MW = 43 kDa. This protein runs at a higher M.W. by SDS-PAGE due to glycosylation. This protein is enzymatically biotinylated using Avi-Tag™ technology. Biotinylation is confirmed to be ≥90%. VHHs are single-domain antibodies from camelids.

Format: Aqueous buffer solution

Formulation: 8 mM Phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol.

Purification: Protein A Chromatography

Purity: ≥90%

Storage Stability: Stable for at least 6 months at -80°C.

Supplied As: Aqueous buffer solution.

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: 1. Ho, M. et al., Antibody Therapeutics. 2020; 3(2): 109-114
2. Wrapp, D. et al., 2020. Cell 181: 1004-1015

• Artikelnummer: BPS100785-1
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 100785-1
• Green Labware: Nein
• Verpackungseinheit: 50 µg
• Mengeneinheit: STK
• Klonalität: Monoclonal
• Methode: ELISA
• Isotyp: IgG1