Spike Trimer (S1+S2), His-tag, Eu-labeled (SARS-CoV-2) Recombinant

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Encompassing Amino Acids: 16-1213

Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 

Assay Conditions: SARS-CoV2-Trimer Eu-labeled protein (6.25 nM) was incubated with ACE2, His-Avi-Tag, Biotin-labeled HiP (BPS Bioscience, #100665) and the Dye-labeled acceptor in ACE2-Spike TR-FRET buffer for 1 hour at room temperature. TR-FRET signals were measured and the ratio was calculated as [Ex340nm/Em665nm] /[Ex340nm/Em620nm].

Description: Severe acute respiratory Coronavirus Spike trimer (S1+S2), Genbank Accession No. MN908947, a.a. 16-1213, with a with C-terminal His-tag, Eu-labeled, expressed in a HEK293 expression system. MW=139 kDa. This protein runs at a higher M.W. by SDS-PAGE due to glycosylation.

Format: Aqueous buffer solution.

Formulation: 40 mM Tris pH 8.0, 110 mM NaCl, 2.2 mM KCl, 200 mM Imidazole and 20% glycerol.

Genbank: MN908947

Purity: >90%

Storage Stability: At least 6 months at -80°C.

Supplied As: Aqueous buffer solution.

Tags: C-terminal His-tag

Uniprot: P0DTC2

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: 1. Klumpp-Thomas, C., et al., MedRxiv. 2020 Jul; preprint doi: 10.1101/2020.07.08.20147371
2. Yurkovetskiy, L., et al., bioRxiv. 2020 Jul; preprint doi: 10.1101/2020.07.04.187757

• Artikelnummer: BPS100894
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 100894
• Green Labware: Nein
• Verpackungseinheit: 25 µg
• Mengeneinheit: STK