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Spike Trimer (S1+S2) (XBB.1.31, Omicron Variant), His-Tag (SARS-CoV-2) Recombinant
Spike Trimer (S1+S2) (XBB.1.31, Omicron Variant), His-Tag (SARS-CoV-2) Recombinant
Artikelnummer
BPS101816-2
Verpackungseinheit
100 µg
Hersteller
BPS Bioscience
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Applications: Screen or titrate anti-spike antibodies or inhibitors; use as antigen control in assay design and optimization.
Assay Conditions: The ELISA was performed by coating 100 µl of ACE2-Fc at 2 µg/ml in PBS at 4ËšC overnight. The plate was washed in PBS and blocked with 200 µl of 1% BSA in PBS for 1 hour at room temperature. Increasing amounts of Spike Trimer XBB.1.31 were added to the plate (serially diluted in blocking buffer). The plate was washed in PBS before adding 100 µl of AffiniPure Peroxidase-conjugated Anti-His-Tag antibody (Jackson Immuno Research #300-035-240) diluted 1:5,000 in blocking buffer for 1 hour at room temperature. The plate was washed with PBS and 100 µl of MB/E Ultra-Sensitiv HRP Substrate (EMD Millipore #ES022-500ml) was added for 5 minutes at room temperature. The reaction was stopped with 100 µl of 0.6N H2SO4 and read at 450 nm.
Description: Recombinant SARS-CoV-2 Spike protein in its homotrimeric form, containing S1+S2 subunits. This protein corresponds to SARS-CoV-2 Omicron Variant XBB.1.31 and contains the Omicron Spike mutations listed below. The construct also contains a C-terminal His-tag. The recombinant protein was affinity purified.
Format: Aqueous buffer solution
Formulation: 1X PBS, 0.025% NaN3
Host Cell Line: HEK293
Storage Stability: At least 6 months at -80°C. This protein is highly sensitive to freeze/thaw cycles.
Tags: C-terminal His-tag
Biosafety Level: Not applicable (BSL-1)
Applications: Screen or titrate anti-spike antibodies or inhibitors; use as antigen control in assay design and optimization.
Assay Conditions: The ELISA was performed by coating 100 µl of ACE2-Fc at 2 µg/ml in PBS at 4ËšC overnight. The plate was washed in PBS and blocked with 200 µl of 1% BSA in PBS for 1 hour at room temperature. Increasing amounts of Spike Trimer XBB.1.31 were added to the plate (serially diluted in blocking buffer). The plate was washed in PBS before adding 100 µl of AffiniPure Peroxidase-conjugated Anti-His-Tag antibody (Jackson Immuno Research #300-035-240) diluted 1:5,000 in blocking buffer for 1 hour at room temperature. The plate was washed with PBS and 100 µl of MB/E Ultra-Sensitiv HRP Substrate (EMD Millipore #ES022-500ml) was added for 5 minutes at room temperature. The reaction was stopped with 100 µl of 0.6N H2SO4 and read at 450 nm.
Description: Recombinant SARS-CoV-2 Spike protein in its homotrimeric form, containing S1+S2 subunits. This protein corresponds to SARS-CoV-2 Omicron Variant XBB.1.31 and contains the Omicron Spike mutations listed below. The construct also contains a C-terminal His-tag. The recombinant protein was affinity purified.
Format: Aqueous buffer solution
Formulation: 1X PBS, 0.025% NaN3
Host Cell Line: HEK293
Storage Stability: At least 6 months at -80°C. This protein is highly sensitive to freeze/thaw cycles.
Tags: C-terminal His-tag
Biosafety Level: Not applicable (BSL-1)
| Artikelnummer | BPS101816-2 |
|---|---|
| Hersteller | BPS Bioscience |
| Hersteller Artikelnummer | 101816-2 |
| Green Labware | Nein |
| Verpackungseinheit | 100 µg |
| Mengeneinheit | STK |
| Wirt | Human |
| Produktinformation (PDF) | Download |
| MSDS (PDF) |
|
Immer für Sie da
Gernot Ensinger, M.Sc.
Laurent Haze
