Products from BPS Bioscience require a minimum order value above 400€
Encompassing Amino Acids: 2-492(end)
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Enzyme reaction is conducted in a buffer containing 50 mM HEPES (pH 7.5), 10 mM MgCl2, 1 mM EGTA, 200 µM ATP, 0.01% Brij-35 and 2 µM substrate (Ser/Thr Peptide 7, Invitrogen) at 30°C for 1 hour.
Background: STK3, also known as MST2, encodes a protein of 491-amino acid which contains an N-terminal catalytic domain characteristic of STKs. STK3 and STK4 share 94% amino acid identity in the catalytic domain and 78% identity overall. RAF1 has been shown to counteract apoptosis by suppressing the activation of mammalian sterile 20-like kinase (MST2). STK3 is a kinase that is activated by the proapoptotic agents straurosporine and FAS ligand. STK3 activation presumably allows cells to resist unfavorable environmental conditions.
Description: Human STK3 (serine/threonine kinase 3), also known as MST-2 (Mammalian STE20-like protein kinase 2) or KRS1, GenBank Accession No. NM_006281, a.a. 2-492(end), with N-terminal GST-tag, MW=78.4 kDa, expressed in Sf9 insect cells via a baculovirus expression system.
Format: Aqueous buffer solution
Formulation: 45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 3 mM DTT, 18 mM glutathione, and 10% glycerol
Genbank: NM_006281
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: Q13188
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Taylor, L.K., et al. (1996). Proc. Natl. Acad. Sci. USA. 93(19):10099-104.
2. Song, H., et al. (2010). Proc. Natl. Acad. Sci. USA. 107(4):1431-6.
3. Kim, D., et al. (2010). PLoS One 5(3):e9616.