SUV39H2, GST-Tag Recombinant

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Encompassing Amino Acids: 26-350

Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 

Assay Conditions: 50 µl reaction mix (50 mM Tris pH 8.8, 5 mM MgCl₂, 4 mM DTT, 20 µM S-adenosylmethionine, and 0-40 ng SUV39H2) is added to the wells of a substrate-coated plate. Incubate for 1 hr. Add antibody against methylated K9 residue of histone H3, incubate 1 hr.Finally, incubate 1 hr. with the Europium-labeled secondaryantibody. Time resolved fluorescencemeasured at room temperature (?exc=330nm, ?em=620 nm).

Description: Human SUV39H2, GenBank Accession No. NM_024670, a.a. 26-350, with N-terminal GST-tag, MW= 63 kDa, expressed in an E. coli expression system.

Format: Aqueous buffer solution

Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 16 mM glutathione

Genbank: NM_024670

Host Cell Line: E. coli

Storage Stability: At least 6 months at -80°C.

Tags: N-terminal GST-tag

Uniprot: Q9H5I1

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: 1. Yoon,K.A. et al., Carcinogenesis 27, 11, 2217-2222, 2006.
2. Frontelo, P. et al., Oncogene 23, 30, 5242- 5251, 2004.

• Artikelnummer: BPS51080
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 51080
• Green Labware: Nein
• Verpackungseinheit: 50 µg
• Mengeneinheit: STK