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Encompassing Amino Acids: 1001-1327(end)
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Enzyme reaction is conducted using BPS's TNKS1 HistoneRibosylation Assay Kit (Biotin-labeled NAD+),Catalog # 80573.
Description: TNKS1 (TRF1-interacting ankyrin-related ADP-ribose polymerase 1), also known as PARP5A, TANK1, or tanykyrase-1 (GenBank Accession No. NM_003747), a.a. 1001-1327(end), with N-terminal GST-tag, MW=62.5 kDa, expressed in Sf9 cells via a baculovirus expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris pH 8.0, 110 mM NaCl, 2.2 mM KCl, 20% glycerol, and 3 mM DTT
Genbank: NM_003747
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: O95271
Unit Definition: One unit of PARP incorporates 100 pmoles of poly(ADP) in 1 minute from NAD into the acid-insoluble form.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Chang S. (2010). Aging (Albany NY). 2(10):639-42.
2. Karner, C.M. et al. (2010). Dev Dyn. 239(7):2014-23.
3. McCabe, N, et al. (2009). Oncogene. 28(11):1465-70.